Laboratory Studies
- Culture of the tongue's dorsal surface may be taken if a superimposed oral candidiasis or other specific oral infection is suspected.
- Cytologic smears stained with Gram stain or periodic acid-Schiff stain may reveal candidal organisms.
- Potassium hydroxide preparations are useful for rapid diagnosis of oral candidiasis, as are latex agglutination-based card tests (eg, CandidaSure).
Procedures
- Distinguishing between oral hairy leukoplakia and hairy tongue is important if patients are found or suspected to be HIV positive. This can be accomplished by a simple mucosal punch biopsy and appropriate immunostaining of the specimen for the presence of Epstein-Barr virus, the causative agent of oral hairy leukoplakia. However, in most cases, the diagnosis is made retrospectively on the basis of the clinical response to mechanical debridement.[6]
Histologic Findings
Histopathologic findings in hairy tongue consist of elongated filiform papillae, with mild hyperkeratosis and occasional inflammatory cells. Finding accumulated debris intermingled among the papillae and candidal pseudohyphae is not unusual. No other specific microscopic findings are associated with this entity.
Garg A, Wadhera R, Gulati SP, Goyal R. Hairy tongue. J Assoc Physicians India. Oct 2008;56:817-8. [Medline].
Vañó-Galván S, Jaén P. Black hairy tongue. Cleve Clin J Med. Dec 2008;75(12):847-8. [Medline].
Lawoyin D, Brown RS. Drug-induced black hairy tongue: diagnosis and management challenges. Dent Today. Jan 2008;27(1):60, 62-3; quiz 93, 58. [Medline].
Pigatto PD, Spadari F, Meroni L, Guzzi G. Black hairy tongue associated with long-term oral erythromycin use. J Eur Acad Dermatol Venereol. Nov 2008;22(10):1269-70. [Medline].
Thompson DF, Kessler TL. Drug-induced black hairy tongue. Pharmacotherapy. Jun 2010;30(6):585-93. [Medline].
Rushing EC, Hoschar AP, McDonnell JK, Billings SD. Iatrogenic oral hairy leukoplakia: report of two cases. J Cutan Pathol. Mar 2011;38(3):275-9. [Medline].
Kostka E, Wittekindt C, Guntinas-Lichius O. [Tongue coating, mouth odor, gustatory sense disorder - earlier and new treatment options by means of tongue scraper]. Laryngorhinootologie. Aug 2008;87(8):546-50. [Medline].
[Guideline] New York State Department of Health. Oral health management of children and adolescents with HIV infections. New York State Department of Health. Jun 2004.
[Guideline] Infectious Diseases Society of America. Clinical practice guidelines for the management of candidiasis: 2009 update by the Infectious Diseases Society of America. Infectious Diseases Society of America. Mar 2009.
[Guideline] Singapore Ministry of Health. Nursing management of oral hygiene. Singapore Ministry of Health. Dec 2004.
Bouquot JE. Common oral lesions found during a mass screening examination. J Am Dent Assoc. Jan 1986;112(1):50-7. [Medline].
Christen AG, Swanson BZ Jr. Oral hygiene: a history of tongue scraping and brushing. J Am Dent Assoc. Feb 1978;96(2):215-9. [Medline].
Darwazeh AM, Pillai K. Prevalence of tongue lesions in 1013 Jordanian dental outpatients. Community Dent Oral Epidemiol. Oct 1993;21(5):323-4. [Medline].
Donta AN, Lampadakis J, Pilalitos P, Spyropoulos ND. [Findings from the clinical examination of the oral cavity of one hundreddrug addicts]. Hell Stomatol Chron. Apr-Jun 1989;33(2):101-5. [Medline].
Farman AG. Hairy tongue (lingua villosa). J Oral Med. Jul-Sep 1977;32(3):85-91. [Medline].
Hasler JF, Standish SM. Podophyllin treatment of hairy tongue: a warning. J Am Dent Assoc. Mar 1969;78(3):563-7. [Medline].
Heymann WR. Psychotropic agent-induced black hairy tongue. Cutis. Jul 2000;66(1):25-6. [Medline].
Jarvinen J, Kullaa-Mikkonen A, Kotilainen R. Some local and systemic factors related to tongue inflammation. Proc Finn Dent Soc. 1989;85(3):199-209. [Medline].
Kaplan I, Moskona D. A clinical survey of oral soft tissue lesions in institutionalized geriatric patients in Israel. Gerodontology. Summer 1990;9(2):59-62. [Medline].
Kullaa-Mikkonen A, Mikkonen M, Kotilainen R. Prevalence of different morphologic forms of the human tongue in young Finns. Oral Surg Oral Med Oral Pathol. Feb 1982;53(2):152-6. [Medline].
Langtry JA, Carr MM, Steele MC, Ive FA. Topical tretinoin: a new treatment for black hairy tongue (lingua villosa nigra). Clin Exp Dermatol. May 1992;17(3):163-4. [Medline].
Levine N. Dark discoloration of the tongue. Geriatrics. Jun 1996;51(6):20. [Medline].
Manabe M, Lim HW, Winzer M, Loomis CA. Architectural organization of filiform papillae in normal and black hairy tongue epithelium: dissection of differentiation pathways in a complex human epithelium according to their patterns of keratin expression. Arch Dermatol. Feb 1999;135(2):177-81. [Medline].
McGregor JM, Hay RJ. Oral retinoids to treat black hairy tongue. Clin Exp Dermatol. May 1993;18(3):291. [Medline].
Nally F. Diseases of the tongue. Practitioner. Jan 1991;235(1498):65-71. [Medline].
Neville BW, Damm DD, Allen CM. Oral and Maxillofacial Pathology. 2nd ed. Philadelphia, Pa: WB Saunders; 2002.
Newman CC, Wagner RF. Images in clinical medicine. Black hairy tongue. N Engl J Med. Sep 25 1997;337(13):897. [Medline].
Pegum JS. Urea in the treatment of black hairy tongue. Br J Dermatol. Jun 1971;84(6):602. [Medline].
Powell FC. Glossodynia and other disorders of the tongue. Dermatol Clin. Oct 1987;5(4):687-93. [Medline].
Redman RS. Prevalence of geographic tongue, fissured tongue, median rhomboid glossitis, and hairy tongue among 3,611 Minnesota schoolchildren. Oral Surg Oral Med Oral Pathol. Sep 1970;30(3):390-5. [Medline].
Regezi JA, Sciubba JJ, Jordan RCK. Oral Pathology - Clinical Pathologic Correlations. 5th ed. Philadelphia, Pa: WB Saunders; 2003.
Salonen L, Axell T, Hellden L. Occurrence of oral mucosal lesions, the influence of tobacco habits and an estimate of treatment time in an adult Swedish population. J Oral Pathol Med. Apr 1990;19(4):170-6. [Medline].
Sapp JP, Eversole LR, Wysocki GP. Contemporary Oral and Maxillofacial Pathology. 2nd ed. St. Louis, Mo: Mosby-Year Book; 2004.
Sarti GM, Haddy RI, Schaffer D, Kihm J. Black hairy tongue. Am Fam Physician. Jun 1990;41(6):1751-5. [Medline].
Winzer M, Gilliar U. [Hairy tongue and hairy oral leukoplakia--a differential histopathologic diagnosis]. Z Hautkr. Jun 15 1988;63(6):517-20. [Medline].
Winzer M, Gilliar U, Ackerman AB. Hairy lesions of the oral cavity. Clinical and histopathologic differentiation of hairy leukoplakia from hairy tongue. Am J Dermatopathol. Apr 1988;10(2):155-9. [Medline].

