Ophthalmologic Manifestations of Escherichia Coli Workup

  • Author: Donny W Suh, MD, FAAP; Chief Editor: Hampton Roy Sr, MD   more...
 
Updated: Mar 9, 2012
 

Laboratory Studies

The following studies are indicated in patients with suspected E coli infection:

  • CBC count with cell differentials
  • Blood agar
  • Sabouraud dextrose agar without cycloheximide
  • Thioglycolate broth
  • Chocolate agar
  • Löwenstein-Jensen medium
  • Nonnutrient agar with E coli overlay
  • Slides - Gram stain, Giemsa stain
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Imaging Studies

CT scanning of the orbit and head may be helpful in case of trauma to look for a foreign body.

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Procedures

With a corneal ulcer, anesthetize the cornea with topical drops, and, at the slit lamp, scrape the base and the leading edge of the infiltrate with the Kimura spatula, and place on the culture medium or slide.

With endophthalmitis, diagnostic and therapeutic vitrectomy is used to confirm the diagnosis and to evaluate the organisms' sensitivity to antibacterial agents.

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Histologic Findings

E coli is a gram-negative rod. E coli has a fermentative and respiratory type of metabolism.

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Contributor Information and Disclosures
Author

Donny W Suh, MD, FAAP  Clinical Assistant Professor, Pediatric Ophthalmology Service, University of Nebraska Medical Center; Pediatric Ophthalmologist, Adult Strabismus Specialist, Wolfe Eye Clinic, PC; Consulting Staff, Blank Children's Hospital, Mercy Medical Center of Des Moines, Iowa Methodist Hospital of Des Moines, and Marshalltown Medical Center

Donny W Suh, MD, FAAP is a member of the following medical societies: American Academy of Ophthalmology, American Academy of Pediatrics, and American Medical Association

Disclosure: Alcon Honoraria Speaking and teaching

Specialty Editor Board

Kilbourn Gordon III, MD, FACEP  Urgent Care Physician

Kilbourn Gordon III, MD, FACEP is a member of the following medical societies: American Academy of Ophthalmology and Wilderness Medical Society

Disclosure: Nothing to disclose.

Francisco Talavera, PharmD, PhD  Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Medscape Salary Employment

J James Rowsey, MD  Former Director of Corneal Services, St Luke's Cataract and Laser Institute

J James Rowsey, MD is a member of the following medical societies: American Academy of Ophthalmology, American Association for the Advancement of Science, American Medical Association, Association for Research in Vision and Ophthalmology, Florida Medical Association, Pan-American Association of Ophthalmology, Sigma Xi, and Southern Medical Association

Disclosure: Nothing to disclose.

Lance L Brown, OD, MD  Ophthalmologist, Affiliated With Freeman Hospital and St John's Hospital, Regional Eye Center, Joplin, Missouri

Disclosure: Nothing to disclose.

Chief Editor

Hampton Roy Sr, MD  Associate Clinical Professor, Department of Ophthalmology, University of Arkansas for Medical Sciences

Hampton Roy Sr, MD is a member of the following medical societies: American Academy of Ophthalmology, American College of Surgeons, and Pan-American Association of Ophthalmology

Disclosure: Nothing to disclose.

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