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Pericholangitis Workup

  • Author: Robert P Myers, MD, FRCPC; Chief Editor: BS Anand, MD  more...
Updated: Jun 22, 2016

Laboratory Studies

Liver enzymes (alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, and gamma-glutamyl transpeptidase) should be assessed. Patients typically have a cholestatic liver profile with predominant elevations of alkaline phosphatase and gamma-glutamyl transpeptidase. Aminotransferase levels may be within the reference range or be moderately elevated.

Serum bilirubin levels may range from within the reference range to displaying marked elevation. The bilirubin level may rise insidiously in association with gradual progression of the disease or rise abruptly with intermittent episodes of fever and abdominal pain suggesting acute cholangitis.

In the early stages of the disease, the prothrombin time (PT) and the albumin level typically are within the reference ranges, although the albumin level may be decreased as a consequence of coexisting inflammatory bowel disease (IBD). As the disease progresses to later stages, the PT may rise and the albumin may fall.

Patients may have coexistent risk factors for the acquisition of viral hepatitis, including the blood transfusions that are often necessary for the management of anemia in patients with IBD. Rule out chronic hepatitis B and C with hepatitis B surface antigen (HBsAg) and antihepatitis C (anti-HCV) antibody tests. Hypergammaglobulinemia and hypercholesterolemia have been reported in some patients.

The perinuclear antineutrophil cytoplasmic antibody (pANCA) test reveals a reported prevalence of 26-85% in classic primary sclerosing cholangitis (PSC). In one study, the sensitivity and specificity of pANCA testing with the immunofluorescence technique were 51% and 73%, respectively. The usefulness of the pANCA test in pericholangitis is not clear.

Antimitochondrial antibody testing should be ordered to rule out primary biliary cirrhosis (PBC), a common cause of cholestatic liver disease. Patients with pericholangitis characteristically lack this antibody.

Antinuclear antibodies (ANA), smooth muscle antibodies (SMA), or both have been reported in 28% of patients with pericholangitis. Tests for these antibodies should be ordered to rule out autoimmune hepatitis as a cause of liver biochemical abnormalities, although this condition typically presents with elevated levels of aminotransferases rather than a cholestatic liver profile.

Autoantigen testing may prove useful in this setting. A study by Ardesjo et al reported the identification of a novel staining pattern of bile duct epithelial cells in patients with PSC, which appeared to have potential utility as an early diagnostic marker.[11]

The investigators used immunostaining on normal human bile duct with sera from affected patients and control patients, then constructed a cDNA library from normal human bile duct and immunoscreened patient sera to identify an autoantigen.[11] Finally, using in vitro transcription and translation and immunoprecipitation, they assessed immunoreactivity against PDZ domain containing 1 (PDZK1) in patients with PSC (n = 35), control patients (n = 198), and healthy control subjects (n = 94).

The PSC sera stained cytoplasmic granules and apical cell membranes of biliary epithelial cells in a novel staining pattern.[11] Strong immunoreactivity to these structures were found in 12 of 35 (34%) PSC sera but not in sera from the healthy control subjects.[11] When the cDNA library was screened, PDZK1 was identified as a potential new autoantigen. Strong immunoreactivity to PDZK1 was detected in 1% of healthy controls, 2% of IBD patients, 8% of autoimmune pancreatitis patients, 9% of PSC patients, and 18% of Graves disease patients.

One study suggests that U2 small nuclear RNA fragments in the bile fluid may be a potential diagnostic marker for cholangiocarcinoma. Baraniskin et al reported that significantly higher bile levels of U2 RNA fragments in those with cholangiocarcinoma allowed differentiation from PSC, as measured by real-time PCR normalized to cel-54 (67% sensitivity, 91% specificity).[12]


Abdominal Ultrasonography

No specific ultrasonographic findings exist for the diagnosis of pericholangitis. Perform abdominal ultrasonography to screen for other causes of hepatic pathology, including fatty liver and cholelithiasis, which also may affect patients with IBD. Although ultrasonography is insensitive, it may detect findings suggestive of cirrhosis such as a nodular, shrunken liver, or portal hypertensive changes, including splenomegaly, altered portal venous blood flow, or the formation of venous collaterals.



Perform endoscopic retrograde cholangiopancreatography (ERCP) to rule out large-duct PSC, a much more common cause of cholestasis in patients with IBD. Findings include stricturing and beading of the intrahepatic or extrahepatic bile ducts. By definition, ERCP findings are normal in patients with pericholangitis. However, some patients with large-duct PSC may have histologic evidence of pericholangitis or small-duct disease.

The role of magnetic resonance cholangiopancreatography (MRCP) in pericholangitis is unclear. Preliminary studies suggest that MRCP may be as useful as ERCP in detecting the typical cholangiographic features of PSC. MRCP findings are normal in patients with pericholangitis.


Liver Biopsy

Performing a liver biopsy is essential for the diagnosis and staging of pericholangitis and to rule out other causes of liver disease.

Bleeding is the predominant risk associated with liver biopsy. Fatal and nonfatal hemorrhages have been reported in 0.04% and 0.16% of patients, respectively. Ultrasound guidance may reduce the risk of complications.

Histologic Findings

Pericholangitis may be classified into 4 stages on the basis of histologic findings. The histologic stage is an important predictor of survival in PSC and likely bears the same importance in pericholangitis.

Stage 1 (portal stage) is characterized as follows:

  • Lymphocytic portal inflammation, accompanied with variable amounts of edema, fibrosis, and bile ductular proliferation
  • Most ducts are without an abnormality, but some show degenerative changes, periductal fibrosis, and nonsuppurative fibrous cholangitis (the typical “onion-skin lesion” of PSC)

Stage 2 (periportal stage) is characterized as follows:

  • Periportal fibrosis with or without periportal hepatitis, and expansion of the portal triads with seemingly intact newly formed limiting plates
  • Focal ductopenia often is noted in this stage

Stage 3 (septal stage) is characterized as follows:

  • Septal fibrosis, bridging necrosis, or both are prominent
  • Bile ducts often are severely damaged or absent
  • Other features include piecemeal necrosis and prominent copper deposition

Stage 4 (cirrhotic stage) is characterized as follows:

  • The liver is cirrhotic, and bile ducts often have disappeared
  • At this stage, differentiation from other cholestatic liver diseases, particularly primary biliary cirrhosis (PBC), may be difficult
Contributor Information and Disclosures

Robert P Myers, MD, FRCPC Assistant Professor, Director, Viral Hepatitis Clinic, Division of Gastroenterology, Department of Medicine, University of Calgary Faculty of Medicine, Canada

Robert P Myers, MD, FRCPC is a member of the following medical societies: American Association for the Study of Liver Diseases, Royal College of Physicians and Surgeons of Canada, Canadian Association of Gastroenterology

Disclosure: Nothing to disclose.


Ali Nawaz Khan, MBBS, FRCS, FRCP, FRCR Consultant Radiologist and Honorary Professor, North Manchester General Hospital Pennine Acute NHS Trust, UK

Ali Nawaz Khan, MBBS, FRCS, FRCP, FRCR is a member of the following medical societies: American Association for the Advancement of Science, American Institute of Ultrasound in Medicine, British Medical Association, Royal College of Physicians and Surgeons of the United States, British Society of Interventional Radiology, Royal College of Physicians, Royal College of Radiologists, Royal College of Surgeons of England

Disclosure: Nothing to disclose.

Chief Editor

BS Anand, MD Professor, Department of Internal Medicine, Division of Gastroenterology, Baylor College of Medicine

BS Anand, MD is a member of the following medical societies: American Association for the Study of Liver Diseases, American College of Gastroenterology, American Gastroenterological Association, American Society for Gastrointestinal Endoscopy

Disclosure: Nothing to disclose.

Additional Contributors

Julian Katz, MD Clinical Professor of Medicine, Drexel University College of Medicine

Julian Katz, MD is a member of the following medical societies: American College of Gastroenterology, American College of Physicians, American Gastroenterological Association, American Geriatrics Society, American Medical Association, American Society for Gastrointestinal Endoscopy, American Society of Law, Medicine & Ethics, American Trauma Society, Association of American Medical Colleges, Physicians for Social Responsibility

Disclosure: Nothing to disclose.


Tushar Patel, MB, ChB Professor of Medicine, Ohio State University Medical Center

Tushar Patel, MB, ChB is a member of the following medical societies: American Association for the Study of Liver Diseases and American Gastroenterological Association

Disclosure: Nothing to disclose.

Francisco Talavera, PharmD, PhD Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Medscape Salary Employment

Noel Williams, MD Professor Emeritus, Department of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada; Professor, Department of Internal Medicine, Division of Gastroenterology, University of Alberta, Edmonton, Alberta, Canada

Noel Williams, MD is a member of the following medical societies: Royal College of Physicians and Surgeons of Canada

Disclosure: Nothing to disclose.

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