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Ebola Virus Infection

  • Author: John W King, MD; Chief Editor: Pranatharthi Haran Chandrasekar, MBBS, MD  more...
 
Updated: Jun 03, 2016
 

Practice Essentials

Ebola virus (see the image below) is one of at least 30 known viruses capable of causing viral hemorrhagic fever syndrome. The genus Ebolavirus is currently classified into 5 separate species: Sudan ebolavirus, Zaire ebolavirus, Tai Forest (Ivory Coast) ebolavirus, Reston ebolavirus, and Bundibugyo ebolavirus. The 2014 outbreak of Ebola virus disease in West Africa, involving Zaire ebolavirus, is the largest outbreak of Ebola virus disease in history.

Ebola virus. Courtesy of the US Centers for Diseas Ebola virus. Courtesy of the US Centers for Disease Control and Prevention.

For Medscape’s special coverage of the expanding 2014 Ebola virus outbreak, see the Ebola Resource Center. Information on Ebola treatment, recommendations, and safeguards for healthcare personnel can be found in the Critical Images slideshow presentation Ebola: Care, Recommendations, and Protecting Practitioners.

Also see the slideshow 11 Travel Diseases to Consider Before and After the Trip to help identify and manage several infectious travel diseases.

Finally, the CDC has issued a presentation resource for US healthcare workers, available as a Powerpoint file. It is updated weekly (every Friday).

Signs and symptoms

The following 2 types of exposure history are recognized:

  • Primary exposure – This typically involves travel to or work in an Ebola-endemic area
  • Secondary exposure – This refers to human-to-human exposure (eg, medical caregivers, family caregivers, or persons who prepared deceased patients for burial), primate-to-human exposure (eg, animal care workers who provide care for primates), or persons who collect or prepare bush meat for human consumption

Physical findings depend on the stage of disease at the time of presentation. With African-derived Ebolavirus infection, there is an incubation period (typically 3-8 days in primary cases and slightly longer in secondary cases).

Early findings may include the following:

  • Fever
  • Pharyngitis
  • Severe constitutional signs and symptoms
  • Maculopapular rash (best seen in white patients)
  • Bilateral conjunctival injection

Later findings may include the following:

  • Expressionless facies
  • Bleeding from intravenous (IV) puncture sites and mucous membranes
  • Myocarditis and pulmonary edema
  • In terminally ill patients, tachypnea, hypotension, anuria, and coma

Survivors of Ebola virus disease have developed the following late manifestations:

  • Myalgias
  • Asymmetric and migratory arthralgias
  • Headache
  • Fatigue
  • Bulimia
  • Amenorrhea
  • Hearing loss
  • Tinnitus
  • Unilateral orchitis
  • Suppurative parotitis

See Clinical Presentation for more detail.

Diagnosis

Diagnostic studies that may be helpful include the following:

  • Basic blood tests – Complete blood count (CBC) with differential, bilirubin, liver enzymes, blood urea nitrogen (BUN), creatinine, pH
  • Studies for isolating the virus – Tissue culture (only to be performed in one of a few high-containment laboratories throughout the world), reverse-transcription polymerase chain reaction (RT-PCR) assay
  • Serologic testing – Enzyme-linked immunosorbent assay (ELISA) for antigens or for immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies
  • Other studies – Immunochemical testing of postmortem skin, electron microscopy

See Workup for more detail.

Management

General principles of care are as follows:

  • Supportive therapy with attention to intravascular volume, electrolytes, nutrition, and comfort care is of benefit to the patient
  • Such therapy must be administered with strict attention to barrier isolation; all body fluids contain infectious virions and should be handled with great care
  • No specific therapy is available that has demonstrated efficacy in the treatment of Ebola hemorrhagic fever
  • There are no commercially available Ebola vaccines; however, neutralizing antibodies have been studied that may be useful in vaccine development or as passive prophylactic agents

At present, no specific anti-Ebolavirus agents are available. Agents that have been studied for the treatment or prevention of Ebola virus disease include the following:

  • Ribavirin (possesses no demonstrable anti- Ebolavirus activity in vitro and has failed to protect Ebolavirus -infected primates)
  • Nucleoside analogue inhibitors of S-adenosylhomocysteine hydrolase (SAH)
  • Interferon beta
  • Horse- or goat-derived immune globulins
  • Human-derived convalescent immune globulin preparations
  • Recombinant human interferon alfa-2
  • Recombinant human monoclonal antibody against the envelope glycoprotein (GP) of Ebola virus
  • DNA vaccines expressing either envelope GP or nucleocapsid protein (NP) genes of Ebola virus
  • Activated protein C [1]
  • Recombinant inhibitor of factor VIIa/tissue factor [2]

In those patients who do recover, recovery often requires months, and delays may be expected before full resumption of normal activities. Weight gain and return of strength are slow. Ebola virus continues to be present for many weeks after resolution of the clinical illness.

See Treatment and Medication for more detail.

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Background

Ebola virus is one of at least 30 known viruses capable of causing viral hemorrhagic fever syndrome. (See Pathophysiology and Etiology.) Although agents that cause viral hemorrhagic fever syndrome constitute a geographically diverse group of viruses, all of those identified to date are RNA viruses with a lipid envelope, all are considered zoonoses, all damage the microvasculature (resulting in increased vascular permeability), and all are members of 1 of the following 4 families:

  • Arenaviridae
  • Bunyaviridae
  • Flaviviridae
  • Filoviridae

Although some of the hemorrhagic fever viruses are normally spread by ticks or mosquitoes, all but one (ie, dengue hemorrhagic fever) are capable of being spread by aerosols, and this capability makes these viruses potential bioterrorism agents.

The family Filoviridae resides in the order Mononegavirales and contains the largest genome within the order. This family contains 2 genera: Ebolavirus (containing 5 species) and the antigenically distinct Marburgvirus (containing a single species).

In patients who have Ebola virus infection, exposure to the virus may be either primary (involving presence in an Ebolavirus -endemic area) or secondary (involving human-to-human or primate-to-human transmission). Physical findings depend on the stage of disease at the time of presentation. (See Presentation.)

Studies have demonstrated that patients who die of Ebola viral infection do not develop a humoral immune response. However, in survivors neutralizing antibody can be detected. It is likely that a broad humoral immune response can increase the likelihood of an infected patient surviving Ebola.

Currently, no specific therapy is available that has demonstrated efficacy in the treatment of Ebola hemorrhagic fever, and there are no commercially available Ebola virus vaccines. (See Treatment.) General medical support is critical. Care must be administered with strict attention to barrier isolation. Because the source of Ebola virus is unknown, education and prevention of primary cases is problematic. Education of communities at risk, especially healthcare workers, can greatly reduce the number of secondary person-to-person transmissions.

For information on the current (2014) Ebola virus outbreak, see Epidemiology. The current outbreak has prompted the Centers for Disease Control and Prevention (CDC) to issue a level 3 travel advisory (avoidance of nonessential travel) to Sierra Leone, Guinea, and Liberia; a level 2 travel advisory (practice enhanced precautions) to Democratic Republic of the Congo; and level 1 travel advisory (practice usual precautions) to Nigeria. Those who travel to Sierra Leone, Guinea, and Liberia are advised to avoid contact with the blood and body fluids of people with known Ebola virus infection.[3]

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Pathophysiology and Etiology

Ultrastructure and pathogenesis

The known members of the family Filoviridae are the genera Ebolavirus (Ebola virus) and Marburgvirus (Marburg virus). According the 2012 virus taxonomy of the International Committee on Taxonomy of Viruses, Ebolavirus is classified into the following 5 separate species:

  1. Sudan ebolavirus
  2. Zaire ebolavirus
  3. Tai Forest ebolavirus (formerly and perhaps still more commonly Ivory Coast ebolavirus or Côte d’Ivoire ebolavirus)
  4. Reston ebolavirus
  5. Bundibugyo ebolavirus

Filoviruses such as Ebola virus share a characteristic filamentous form, with a uniform diameter of approximately 80 nm but a highly variable length. Filaments may be straight, but they are often folded on themselves (see the image below).

Ebola virus. Courtesy of the US Centers for Diseas Ebola virus. Courtesy of the US Centers for Disease Control and Prevention.

Ebola virus has a nonsegmented negative-stranded RNA genome containing 7 structural and regulatory genes. The Ebola genome codes for 4 virion structural proteins (VP30, VP35, nucleoprotein, and a polymerase protein [L]) and 3 membrane-associated proteins (VP40, glycoprotein [GP], and VP24). The GP gene is positioned fourth from the 3′ end of the 7 linearly arranged genes.

After infection, human and nonhuman primates experience an early period of rapid viral multiplication that, in lethal cases, is associated with an ineffective immunologic response. Although a full understanding of Ebola virus disease must await further investigations, part of the pathogenesis has been elucidated.

Most filovirus proteins are encoded in single reading frames; the surface GP is encoded in 2 frames (open reading frame [ORF] I and ORF II). The ORF I (amino-terminal) of the gene encodes for a small (50-70 kd), soluble, nonstructural secretory glycoprotein (sGP) that is produced in large quantities early in Ebola virus infection.[4]

The sGP binds to neutrophil CD16b, a neutrophil-specific Fc g receptor III, and inhibits early neutrophil activation. The sGP also may be responsible for the profound lymphopenia that characterizes Ebola infection. Thus, sGP is believed to play pivotal roles in the ability of Ebola to prevent an early and effective host immune response. One hypothesis is that the lack of sGP production by Marburg virus may explain why this agent is less virulent than African-derived Ebola virus.

Leroy et al reported their observations of 24 close contacts of symptomatic patients actively infected with Ebola.[5] Eleven of the 24 contacts developed evidence of asymptomatic infection associated with viral replication. Viral replication was proven by the authors’ ability to amplify positive-stranded Ebola virus RNA from the blood of the asymptomatic contacts.

A detailed study of these infected but asymptomatic individuals revealed that they had an early (4-6 days after infection) and vigorous immunologic response with production of interleukin (IL)–1β, IL-6, and tumor necrosis factor (TNF), resulting in enhanced cell-mediated and humoral-mediated immunity. In patients who eventually died, proinflammatory cytokines were not detected even after 2-3 days of symptomatic infection.

A second, somewhat larger (120-150 kd) GP, transmembrane glycoprotein, is incorporated into the Ebola virion and binds to endothelial cells but not to neutrophils. Ebola virus is known to invade, replicate in, and destroy endothelial cells. Destruction of endothelial surfaces is associated with disseminated intravascular coagulation, and this may contribute to the hemorrhagic manifestations that characterize many, but not all, Ebola infections.

Clinical infection in human and nonhuman primates is associated with rapid and extensive viral replication in all tissues. Viral replication is accompanied by widespread and severe focal necrosis. The most severe necrosis occurs in the liver, and this is associated with the formation of Councilman-like bodies similar to those seen in yellow fever. In fatal infections, the host’s tissues and blood contain large numbers of Ebola virions, and the tissues and body fluids are highly infectious.

The 5 Ebolavirus species were named for the locations where they caused documented human or animal disease. Two African species, Sudan ebolavirus and Zaire ebolavirus, have been responsible for most of the reported deaths. Clinical disease due to African-derived Ebola virus is severe and, with the exception of a patient who survived infection with a third African species, Ivory Coast ebolavirus, is associated with a mortality ranging from 65% (Sudan, 1979) to 89% (Democratic Republic of the Congo [DRC], December 2002 to April 2003).

A fourth Ebolavirus species, Reston ebolavirus, was first isolated in 1989 in monkeys imported from a single Philippine exporter. A virtually identical isolate imported from the same Philippine exporter was detected in 1992 in Siena, Italy. To date, this species has not been documented to cause human disease.

The fifth Ebolavirus species, also of African lineage, is Bundibugyo ebolavirus, which caused an outbreak in Uganda in 2007-2008, with a mortality of 25%.[6]

Between 1994 and 1997, a stable strain of Ebola virus caused 3 successive outbreaks of hemorrhagic fever in Gabon (mortality, 60-74%).[7] Because the Gabon strain shares a greater than 99% homology of the nucleoprotein and GP gene regions with Zaire ebolavirus, it has not been considered a distinct species.

A likely reservoir for filoviruses has been identified. In 1996, members of the National Institute for Virology of South Africa went to Kikwit, DRC, and evaluated the infectivity of Ebola virus for 24 species of plants and 19 species of vertebrates and invertebrates.[8] Insectivorous bats and fruit bats were found to support Ebola virus replication without dying. Furthermore, serum Ebola titers in infected fruit bats reached as high as 106 fluorescent focus-forming units/mL, and feces contained viable Ebola virus.

Mechanisms of dispersion

African-derived filovirus infections are characterized by transmission from an unknown host (possibly bats) to humans or nonhuman primates, presumably via direct contact with body fluids such as saliva or blood or other infected tissues. Evidence in nonhuman primates indicates that Sudan ebolavirus and Zaire ebolavirus may be transmitted by contact with mucous membranes, conjunctiva, pharyngeal and gastrointestinal (GI) surfaces; through small breaks in the skin; and, at least experimentally, by aerosol.[9]

Dogs have been shown to acquire asymptomatic Ebola virus infections, possibly by contact with virus-laden droplets of urine, feces, or blood of unknown hosts.[10] Of epidemiologic significance was the observation that seroprevalence rates in dogs rose in a linear fashion as sampling approached areas of human cases, reaching as high as 31.8%. Thus, an increase in canine seroprevalence may serve as an indicator of increasing Ebola virus circulation in primary vectors within specific geographical areas.

Human infection with African-derived strains has often occurred in caregivers (either family or medical) and in family members who have prepared dead relatives for burial. Late stages of Ebola virus disease are associated with the presence of large numbers of virions in body fluids, tissues, and, especially, skin. Individuals who are exposed to patients infected with Ebola without proper barrier protection are at high risk of becoming infected.

A report from the DRC identified Ebola virus RNA in 100% of oral secretions from patients who had the viral RNA in their serum. Both serum and oral secretions were tested with reverse-transcriptase polymerase chain reaction (RT-PCR) assay. Thus, oral secretions may be capable of transmitting Ebola virus.

The first recorded outbreak occurred in 1976, in Yambuku, DRC, where 316 patients were infected. In the largest recorded urban outbreak to date (DRC, 1995; 318 cases), admission to a hospital greatly amplified the frequency of transmission. The lack of proper barrier protection and the use and reuse of contaminated medical equipment, especially needles and syringes, resulted in rapid nosocomial spread of infection. Only after adequate barrier protection and alteration in burial rituals were implemented was the outbreak contained.

Unlike Asian-derived Ebola virus (ie, Reston ebolavirus, traced to a Philippine supplier of primates), African-derived species appear to be spread more often by direct contact than via the respiratory route. However, the Reston species has repeatedly been demonstrated to spread among nonhuman primates and possibly from primates to humans via the respiratory route. Fortunately, although the Reston species has been documented to be capable of infecting in humans, it does not appear to be pathogenic to humans.

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Epidemiology

Ebola virus is not endemic in the United States, although, during the current (2014) Ebola outbreak, several US healthcare personnel have been infected while in Africa and have been transported to the United States for treatment, in addition to a traveller from Liberia who became ill and sought treatment while visiting Texas. The patient later died of the infection. One of his treating nurses then presented with a low-grade fever and tested positive for Ebola virus infection. In addition, individuals in several US states who recently travelled to West Africa have developed fever and other symptoms, prompting evaluation for Ebola virus infection at US hospitals.[11]

Before the current (2014) outbreak, several human infections with the Reston strain of Ebola had been acquired by animal care workers at primate holding facilities within the United States. Fortunately, the Reston strain has not demonstrated pathogenic effects in humans. Others at potential risk are laboratory workers who work with infected animals or with the virus in tissue culture.

International statistics

Ebola and Marburg viruses are responsible for well-documented outbreaks of severe human hemorrhagic fever, with resultant case mortalities ranging from 23% for Marburg virus to 89% for Ebola virus in which more than one case occurred (see Tables 1, 2, 3, 4, and 5 below).

The current (2014) Ebola virus outbreak is significant and primarily involves 3 African countries—Guinea, Liberia, Sierra Leone. Localized transmission has also been reported in Nigeria. As of October 17, 2014, 8993 total suspected or confirmed cases (5002 laboratory-confirmed) had been reported in these countries, resulting in 4492 deaths. Based on genetic analysis, the virus is 97% identical to the Zaire ebolavirus identified in recent (earlier in 2014) cases in Gabon and the Democratic Republic of the Congo.[12, 13] As of October 8, 2014, 401 healthcare personnel in West Africa had been infected with Ebola, 232 of whom have died.[11] Travel-associated cases have also been reported in Senegal and Spain.[12]

At least 3 Americans in Africa have been infected with Ebola in the current (2014) outbreak, one of whom has died of the disease.[14]

The current outbreak has prompted the CDC to issue a level 3 travel advisory (avoidance of nonessential travel) to Sierra Leone, Guinea, and Liberia; a level 2 travel advisory (practice enhanced precautions) to Democratic Republic of the Congo; and level 1 travel advisory (practice usual precautions) to Nigeria. Those who travel to Sierra Leone, Guinea, and Liberia are advised to avoid contact with the blood and body fluids of people with known Ebola virus infection.[3]

Table 1. History of Sudan Ebola Virus Outbreaks (Open Table in a new window)

Year Location Reported Cases, No. Deaths, No. (%)
1976 Sudan 284 151 (53)
1976 England* 1 0 (0)
1979 Sudan 34 22 (65)
2000-2001 Uganda 425 224 (53)
2004 Sudan 17 7 (41)
2011 Sudan 1 1 (100)
Total   762 405 (53)
Data from Centers for Disease Control and Prevention and World Health Organization.



* Occurred after laboratory accident.



Table 2. History of Zaire Ebola Virus Outbreaks (Open Table in a new window)

Year Location Reported Cases, No. Deaths, No. (%)
1976 Zaire 318 280 (88)
1977 Zaire 1 1 (100)
1994 Gabon 52 31 (60)
1995 DRC 315 250 (81)
Jan 1996 to Apr 1996 Gabon 37 21 (57)
Jul 1996 to Jan 1997 Gabon 60 45 (74)
1996 South Africa (acquired in Gabon) 1 1 (100)
Oct 2001 to Mar 2002 Gabon 65 53 (82)
Oct 2001 to Mar 2002 DRC 59 44 (75)
Dec 2002 to Apr 2003 DRC 143 128 (89)
Nov 2003 to Dec 2004 DRC 35 29 (83)
2007 DRC 264 187 (71)
Dec 2008 to Feb 2009 DRC 32 15 (47)
July 2012 Uganda 24 17 (71)
Nov 2012 DRC 77 36 (46)
Dec 2012 Uganda 7 4 (57)
2014 (as of October 17, 2014) Guinea, Liberia, Sierra Leone, Nigeria, United States, Senegal, Spain (as of October 17, 2014) 8997 (as of October 17, 2014) 4493 (50) (as of October 17, 2014)
Total   10487 5634 (53.7)
Data from Centers for Disease Control and Prevention and World Health Organization.

Table 3. History of Tai Forest (Ivory Coast, Côte-d’Ivoire) Ebola Virus Outbreaks (No Deaths Reported) (Open Table in a new window)

Year Location Reported Cases, No.
1994 Côte-d’Ivoire 1
Total   1
Data from Centers for Disease Control and Prevention and World Health Organization.

Table 4. History of Reston Ebola Virus Outbreaks (No Deaths Reported) (Open Table in a new window)

Year Location Proven * Cases Reported, No.
1989 Virginia, Texas, Pennsylvania 0
1990 Virginia and Texas 4
1989-1990 Philippines 3
1992 Italy 0
1990 Alice, TX 0
1996 Philippines 0
Nov 2008 Philippines 6
Total   13
Data from Centers for Disease Control and Prevention and World Health Organization.



* Humans with serologic evidence of infection but without clinical disease.



Associated with pig farming.[15, 16]



Table 5. History of Bundibugyo Ebola Virus Outbreak (Open Table in a new window)

Year Location Reported Cases, No. Deaths, No. (%)
Dec 2007 to Jan 2008 Uganda 149 37 (25)
Jun to Nov 2012 Democratic Republic of the Congo 36 13 (36.1)
Total   185 50 (27)
Data from Centers for Disease Control and Prevention and World Health Organization.

Individuals considered at risk for Ebola hemorrhagic fever include persons with a travel history to sub-Saharan Africa, persons who have recently cared for infected patients, and animal workers who have worked with primates infected with African-derived Ebola subtypes. In 2011, Uganda experienced a reemergence of the disease.[17]

Age-related demographics

In the 1995 outbreak in Kikwit, DRC, infection rates were significantly lower in children than in adults. During this outbreak, only 27 (8.6%) of the 315 patients diagnosed with Ebola virus infection were aged 17 years or younger. This apparent sparing of children occurs even though 50% of the population of the DRC is younger than 16 years. Although definitive evidence is lacking, epidemiologic evidence suggests that children are less likely to come into direct contact with ill patients than adults are.

Other viral hemorrhagic syndromes, such as Crimean-Congo hemorrhagic fever and hantavirus infections, also show a predominance of adult patients and a relative sparing of young children.

Sex-related demographics

Ebola virus infection has no sexual predilection, but men and women differ with respect to the manner in which direct exposure occurs.

Men, by the nature of their work exposure in forest and savanna regions, may be at increased risk of acquiring a primary infection from gathering “bush meat” (primate carcasses) for food, as well as an unknown vector or vectors. Evidence from Africa and the Philippines is compatible with bats being a principal vector of Ebola virus.

Because women provide much of the direct care for ill family members and are involved in the preparation of the bodies of the deceased, they may be at increased risk of acquiring Ebola virus infection through their participation in these activities. However, men and women who are medical healthcare providers seem to share a high and equal risk of infection.

Race-related demographics

Because most cases of Ebola virus infection have occurred in sub-Saharan Africa, most patients have been black. However, no evidence exists for a specific racial predilection.

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Prognosis

The overall prognosis for patients with Ebola virus infection is poor. However, those who survive for 2 weeks often make a slow recovery.

With the exception of the Reston strain, Ebola virus is associated with very high morbidity and mortality among patients who present with clinical illness, though these vary according to the causative species. The most highly lethal Ebolavirus species is Zaire ebolavirus, which has been reported to have a mortality rate as high as 89%. Sudan ebolavirus also has high reported mortality, ranging from 41% to 65%.

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Contributor Information and Disclosures
Author

John W King, MD Professor of Medicine, Chief, Section of Infectious Diseases, Director, Viral Therapeutics Clinics for Hepatitis, Louisiana State University Health Sciences Center; Consultant in Infectious Diseases, Overton Brooks Veterans Affairs Medical Center

John W King, MD is a member of the following medical societies: American Association for the Advancement of Science, American College of Physicians, American Federation for Medical Research, Association of Subspecialty Professors, American Society for Microbiology, Infectious Diseases Society of America, Sigma Xi

Disclosure: Nothing to disclose.

Coauthor(s)

Hashmi Rafeek, MBBS Fellow in Infectious Diseases, Louisiana State University School of Medicine in Shreveport

Disclosure: Nothing to disclose.

Chief Editor

Pranatharthi Haran Chandrasekar, MBBS, MD Professor, Chief of Infectious Disease, Program Director of Infectious Disease Fellowship, Department of Internal Medicine, Wayne State University School of Medicine

Pranatharthi Haran Chandrasekar, MBBS, MD is a member of the following medical societies: American College of Physicians, American Society for Microbiology, International Immunocompromised Host Society, Infectious Diseases Society of America

Disclosure: Nothing to disclose.

Acknowledgements

Thomas M Kerkering, MD Chief of Infectious Diseases, Virginia Tech Carilion School of Medicine

Thomas M Kerkering, MD is a member of the following medical societies: Alpha Omega Alpha, American College of Physicians, American Public Health Association, American Society for Microbiology, American Society of Tropical Medicine and Hygiene, Infectious Diseases Society of America, Medical Society of Virginia, and Wilderness Medical Society

Disclosure: Nothing to disclose.

Amir A Khan, MD Fellow in Infectious Diseases, Louisiana State University School of Medicine in Shreveport

Amir A Khan, MD is a member of the following medical societies: American College of Physicians and American Medical Association

Disclosure: Nothing to disclose.

Rushdah Malik, MD Fellow, Department of Infectious Diseases, Louisiana State University Health Science Center

Rushdah Malik, MD is a member of the following medical societies: American College of Physicians and Infectious Diseases Society of America

Disclosure: Nothing to disclose.

Francisco Talavera, PharmD, PhD Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Medscape Salary Employment

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Ebola virus. Courtesy of the US Centers for Disease Control and Prevention.
Table 1. History of Sudan Ebola Virus Outbreaks
Year Location Reported Cases, No. Deaths, No. (%)
1976 Sudan 284 151 (53)
1976 England* 1 0 (0)
1979 Sudan 34 22 (65)
2000-2001 Uganda 425 224 (53)
2004 Sudan 17 7 (41)
2011 Sudan 1 1 (100)
Total   762 405 (53)
Data from Centers for Disease Control and Prevention and World Health Organization.



* Occurred after laboratory accident.



Table 2. History of Zaire Ebola Virus Outbreaks
Year Location Reported Cases, No. Deaths, No. (%)
1976 Zaire 318 280 (88)
1977 Zaire 1 1 (100)
1994 Gabon 52 31 (60)
1995 DRC 315 250 (81)
Jan 1996 to Apr 1996 Gabon 37 21 (57)
Jul 1996 to Jan 1997 Gabon 60 45 (74)
1996 South Africa (acquired in Gabon) 1 1 (100)
Oct 2001 to Mar 2002 Gabon 65 53 (82)
Oct 2001 to Mar 2002 DRC 59 44 (75)
Dec 2002 to Apr 2003 DRC 143 128 (89)
Nov 2003 to Dec 2004 DRC 35 29 (83)
2007 DRC 264 187 (71)
Dec 2008 to Feb 2009 DRC 32 15 (47)
July 2012 Uganda 24 17 (71)
Nov 2012 DRC 77 36 (46)
Dec 2012 Uganda 7 4 (57)
2014 (as of October 17, 2014) Guinea, Liberia, Sierra Leone, Nigeria, United States, Senegal, Spain (as of October 17, 2014) 8997 (as of October 17, 2014) 4493 (50) (as of October 17, 2014)
Total   10487 5634 (53.7)
Data from Centers for Disease Control and Prevention and World Health Organization.
Table 3. History of Tai Forest (Ivory Coast, Côte-d’Ivoire) Ebola Virus Outbreaks (No Deaths Reported)
Year Location Reported Cases, No.
1994 Côte-d’Ivoire 1
Total   1
Data from Centers for Disease Control and Prevention and World Health Organization.
Table 4. History of Reston Ebola Virus Outbreaks (No Deaths Reported)
Year Location Proven * Cases Reported, No.
1989 Virginia, Texas, Pennsylvania 0
1990 Virginia and Texas 4
1989-1990 Philippines 3
1992 Italy 0
1990 Alice, TX 0
1996 Philippines 0
Nov 2008 Philippines 6
Total   13
Data from Centers for Disease Control and Prevention and World Health Organization.



* Humans with serologic evidence of infection but without clinical disease.



Associated with pig farming.[15, 16]



Table 5. History of Bundibugyo Ebola Virus Outbreak
Year Location Reported Cases, No. Deaths, No. (%)
Dec 2007 to Jan 2008 Uganda 149 37 (25)
Jun to Nov 2012 Democratic Republic of the Congo 36 13 (36.1)
Total   185 50 (27)
Data from Centers for Disease Control and Prevention and World Health Organization.
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