Rotavirus Workup

  • Author: David D Nguyen, MD, FACEP; Chief Editor: Richard G Bachur, MD   more...
 
Updated: May 22, 2012
 

Laboratory Studies

Rotavirus may be identified by several means (ie, enzyme immunoassay being the most common, latex agglutination, electron microscopy, culture).[3] However, in most cases, identification of the virus is important for public health or infection control purposes.[3] In general, use of such testing is less useful in the ED and can take significant time (days to weeks) for results to return.

This electron micrograph reveals a number of RNA rThis electron micrograph reveals a number of RNA rotavirus virions, and a number of unknown, 29nm virion particles. Image courtesy of Centers for Disease Control and Prevention.

Electrolyte levels should be measured with severe dehydration, alterations in mental status, associated seizures, or oral replenishment with excessive water or salt.

Bedside glucose levels should be measured in very young infants and in any age child with associated lethargy.

 
 
Contributor Information and Disclosures
Author

David D Nguyen, MD, FACEP  Clinical Assistant Professor of Emergency Medicine, The University of Texas Medical Branch at Galveston, TX

David D Nguyen, MD, FACEP is a member of the following medical societies: American College of Emergency Physicians, Harris County Medical Society, and Texas Medical Association

Disclosure: Nothing to disclose.

Coauthor(s)

Sally Henin Awad, MD, FACEP  Medical Director, Forensic Nursing Program, Memorial Hermann Hospital System

Sally Henin Awad, MD, FACEP is a member of the following medical societies: American College of Emergency Physicians, American Medical Writers Association, American Professional Society on the Abuse of Children, and Society for Academic Emergency Medicine

Disclosure: Nothing to disclose.

Brent R King  MD, MMM, Clive Nancy and Pierce Runnells Distinguished Professor of Emergency Medicine; Professor of Pediatrics, University of Texas Health Science Center at Houston; Chair, Department of Emergency Medicine, Chief of Emergency Services, Memorial Hermann Hospital and LBJ Hospital

Brent R King is a member of the following medical societies: American Academy of Emergency Medicine, American Academy of Pediatrics, American College of Emergency Physicians, American College of Physician Executives, and Society for Academic Emergency Medicine

Disclosure: Nothing to disclose.

Specialty Editor Board

Garry Wilkes, MBBS, FACEM  Director of Emergency Medicine, Calvary Hospital, Canberra, ACT; Adjunct Associate Professor, Edith Cowan University, Western Australia

Disclosure: Nothing to disclose.

Mary L Windle, PharmD  Adjunct Associate Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Nothing to disclose.

Grace M Young, MD  Associate Professor, Department of Pediatrics, University of Maryland Medical Center

Grace M Young, MD is a member of the following medical societies: American Academy of Pediatrics and American College of Emergency Physicians

Disclosure: Nothing to disclose.

John D Halamka, MD, MS  Associate Professor of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center; Chief Information Officer, CareGroup Healthcare System and Harvard Medical School; Attending Physician, Division of Emergency Medicine, Beth Israel Deaconess Medical Center

John D Halamka, MD, MS is a member of the following medical societies: American College of Emergency Physicians, American Medical Informatics Association, Phi Beta Kappa, and Society for Academic Emergency Medicine

Disclosure: Nothing to disclose.

Chief Editor

Richard G Bachur, MD  Associate Professor of Pediatrics, Harvard Medical School; Associate Chief and Fellowship Director, Attending Physician, Division of Emergency Medicine, Children's Hospital of Boston

Richard G Bachur, MD is a member of the following medical societies: American Academy of Pediatrics, Society for Academic Emergency Medicine, and Society for Pediatric Research

Disclosure: Nothing to disclose.

References
  1. Turcios RM, Curns AT, Holman RC, et al. Temporal and geographic trends of rotavirus activity in the United States, 1997-2004. Pediatr Infect Dis J. May 2006;25(5):451-4. [Medline].

  2. Gilger MA, Matson DO, Conner ME, Rosenblatt HM, Finegold MJ, Estes MK. Extraintestinal rotavirus infections in children with immunodeficiency. J Pediatr. Jun 1992;120(6):912-7. [Medline].

  3. Cortese MM, Parashar UD. Prevention of rotavirus gastroenteritis among infants and children: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recomm Rep. Feb 6 2009;58:1-25. [Medline]. [Full Text].

  4. FDA Approves New Vaccine to Prevent Gastroenteritis Caused by Rotavirus. US Food and Drug Administration; April 3, 2008. [Full Text].

  5. Yee EL, Staat MA, Azimi P, Bernstein DI, Ward RL, Schubert C, et al. Burden of rotavirus disease among children visiting pediatric emergency departments in Cincinnati, Ohio, and Oakland, California, in 1999-2000. Pediatrics. Nov 2008;122(5):971-7. [Medline].

  6. Linhares AC, Bresee JS. Rotavirus vaccines and vaccination in Latin America. Rev Panam Salud Publica. Nov 2000;8(5):305-31. [Medline].

  7. Christie CD, Duncan ND, Thame KA, Onorato MT, Smith HD, Malcolm LG, et al. Pentavalent rotavirus vaccine in developing countries: safety and health care resource utilization. Pediatrics. Dec 2010;126(6):e1499-506. [Medline].

  8. Rotavirus surveillance--worldwide, 2001-2008. MMWR Morb Mortal Wkly Rep. Nov 21 2008;57(46):1255-7. [Medline]. [Full Text].

  9. Danchin MH, Bines JE. Defeating rotavirus? The global recommendation for rotavirus vaccination. N Engl J Med. Nov 12 2009;361(20):1919-21. [Medline].

  10. Fischer TK, Viboud C, Parashar U, et al. Hospitalizations and deaths from diarrhea and rotavirus among children < 5 years of age in the United States, 1993-2003. J Infect Dis. Apr 15 2007;195(8):1117-25. [Medline].

  11. Newman RD, Grupp-Phelan J, Shay DK, Davis RL. Perinatal risk factors for infant hospitalization with viral gastroenteritis. Pediatrics. Jan 1999;103(1):E3. [Medline].

  12. Anderson EJ, Weber SG. Rotavirus infection in adults. Lancet Infect Dis. Feb 2004;4(2):91-9. [Medline].

  13. Dennehy PH, Cortese MM, Begue RE, et al. A case-control study to determine risk factors for hospitalization for rotavirus gastroenteritis in U.S. children. Pediatr Infect Dis J. Dec 2006;25(12):1123-31. [Medline].

  14. Cezard JP, Bellaiche M, Viala J, Hugot JP. [Medication in infectious acute diarrhea in children]. Arch Pediatr. Oct 2007;14 Suppl 3:S169-75. [Medline].

  15. Martinot A, Pruvost I, Aurel M, Hue V, Dubos F. [Improvement in the management of acute diarrhoea in France?]. Arch Pediatr. Oct 2007;14 Suppl 3:S181-5. [Medline].

  16. Fleisher GR. Infectious disease emergencies. In: Fleisher GR, Ludwig S, Silverman BK, eds. Synopsis of Pediatric Emergency Medicine. 4th ed. Philadelphia, PA: Lippincott Williams and Wilkins; 2002:298-325.

  17. Roslund G, Hepps TS, McQuillen KK. The role of oral ondansetron in children with vomiting as a result of acute gastritis/gastroenteritis who have failed oral rehydration therapy: a randomized controlled trial. Ann Emerg Med. Jul 2008;52(1):22-29.e6. [Medline].

  18. Antiemetics for reducing vomiting related to acute gastroenteritis in children and adolescents [database online]. The Cochrane Library; September 7, 2011.

  19. Turck D. [Prevention and treatment of acute diarrhea in infants]. Arch Pediatr. Nov 2007;14(11):1375-8. [Medline].

  20. Prevention of rotavirus disease: updated guidelines for use of rotavirus vaccine. Pediatrics. May 2009;123(5):1412-20. [Medline].

  21. [Best Evidence] Ruiz-Palacios GM, Perez-Schael I, Velazquez FR, et al. Safety and efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N Engl J Med. Jan 5 2006;354(1):11-22. [Medline].

  22. Cortes JE, Curns AT, Tate JE, Cortese MM, Patel MM, Zhou F, et al. Rotavirus vaccine and health care utilization for diarrhea in US children. N Engl J Med. Sept 22 2011;365:1108-1117.

  23. Dennehy PH, Bertrand HR, Silas PE, Damaso S, Friedland LR, Abu-Elyazeed R. Coadministration of RIX4414 oral human rotavirus vaccine does not impact the immune response to antigens contained in routine infant vaccines in the United States. Pediatrics. Nov 2008;122(5):e1062-6. [Medline].

  24. Global Advisory Committee on Vaccine Safety, December 2011. Wkly Epidemiol Rec. Feb 10 2012;87(6):53-9. [Medline].

  25. Soares-Weiser K, Maclehose H, Bergman H, Ben-Aharon I, Nagpal S, Goldberg E, et al. Vaccines for preventing rotavirus diarrhoea: vaccines in use. Cochrane Database Syst Rev. Feb 15 2012;2:CD008521. [Medline].

  26. Givon-Lavi N, Greenberg D, Dagan R. Comparison between two severity scoring scales commonly used in the evaluation of rotavirus gastroenteritis in children. Vaccine. Oct 29 2008;26(46):5798-801. [Medline].

  27. Information on RotaTeq and Intussusception. US Food and Drug Administration; February 13, 2007. [Full Text].

  28. Patel MM, López-Collada VR, Bulhões MM, De Oliveira LH, Bautista Márquez A, Flannery B, et al. Intussusception risk and health benefits of rotavirus vaccination in Mexico and Brazil. N Engl J Med. Jun 16 2011;364(24):2283-92. [Medline].

  29. Buttery JP, Danchin MH, Lee KJ, Carlin JB, McIntyre PB, Elliott EJ, et al. Intussusception following rotavirus vaccine administration: post-marketing surveillance in the National Immunization Program in Australia. Vaccine. Apr 5 2011;29(16):3061-6. [Medline].

  30. Haber P, Patel M, Izurieta HS, Baggs J, Gargiullo P, Weintraub E, et al. Postlicensure monitoring of intussusception after RotaTeq vaccination in the United States, February 1, 2006, to September 25, 2007. Pediatrics. Jun 2008;121(6):1206-12. [Medline].

  31. Belongia EA, Irving SA, Shui IM, Kulldorff M, Lewis E, Yin R, et al. Real-time surveillance to assess risk of intussusception and other adverse events after pentavalent, bovine-derived rotavirus vaccine. Pediatr Infect Dis J. Jan 2010;29(1):1-5. [Medline].

  32. Zickafoose JS, Benneyworth BD, Riebschleger MP, Espinosa CM, Davis MM. Hospitalizations for Intussusception Before and After the Reintroduction of Rotavirus Vaccine in the United States. Arch Pediatr Adolesc Med. Jan 2 2012;[Medline].

  33. Desai R, Parashar UD, Lopman B, Helena de Oliveira L, Clark AD, Sanderson CF, et al. Potential intussusception risk versus health benefits from rotavirus vaccination in latin america. Clin Infect Dis. May 2012;54(10):1397-405. [Medline].

  34. Information Pertaining to Labeling Revision for RotaTeq. US Food and Drug Administration; June 15, 2007. [Full Text].

  35. Greenberg HB. Rotavirus vaccination and intussusception--act two. N Engl J Med. Jun 16 2011;364(24):2354-5. [Medline].

  36. Tate JE, Cortese MM, Payne DC, Curns AT, Yen C, Esposito DH, et al. Uptake, impact, and effectiveness of rotavirus vaccination in the United States: review of the first 3 years of postlicensure data. Pediatr Infect Dis J. Jan 2011;30(1 Suppl):S56-60. [Medline].

  37. Clark HF, Lawley D, Matthijnssens J, DiNubile MJ, Hodinka RL. Sustained decline in cases of rotavirus gastroenteritis presenting to the Children's Hospital of Philadelphia in the new rotavirus vaccine era. Pediatr Infect Dis J. Aug 2010;29(8):699-702. [Medline].

  38. Wang FT, Mast TC, Glass RJ, Loughlin J, Seeger JD. Effectiveness of the pentavalent rotavirus vaccine in preventing gastroenteritis in the United States. Pediatrics. Feb 2010;125(2):e208-13. [Medline].

  39. Boom JA, Tate JE, Sahni LC, Rench MA, Hull JJ, Gentsch JR, et al. Effectiveness of pentavalent rotavirus vaccine in a large urban population in the United States. Pediatrics. Feb 2010;125(2):e199-207. [Medline].

  40. Yen C, Tate JE, Wenk JD, Harris JM 2nd, Parashar UD. Diarrhea-associated hospitalizations among US children over 2 rotavirus seasons after vaccine introduction. Pediatrics. Jan 2011;127(1):e9-e15. [Medline].

  41. Tate JE, Mutuc JD, Panozzo CA, Payne DC, Cortese MM, Cortes JE, et al. Sustained decline in rotavirus detections in the United States following the introduction of rotavirus vaccine in 2006. Pediatr Infect Dis J. Jan 2011;30(1 Suppl):S30-4. [Medline].

  42. Anderson EJ, Rupp A, Shulman ST, Wang D, Zheng X, Noskin GA. Impact of rotavirus vaccination on hospital-acquired rotavirus gastroenteritis in children. Pediatrics. Feb 2011;127(2):e264-70. [Medline].

  43. Flores AR, Szilagyi PG, Auinger P, Fisher SG. Estimated burden of rotavirus-associated diarrhea in ambulatory settings in the United States. Pediatrics. Feb 2010;125(2):e191-8. [Medline].

  44. Rossignol JF, Abu-Zekry M, Hussein A, Santoro MG. Effect of nitazoxanide for treatment of severe rotavirus diarrhoea: randomised double-blind placebo-controlled trial. Lancet. Jul 8 2006;368(9530):124-9. [Medline].

  45. Rogers M, Weinstock DM, Eagan J, Kiehn T, Armstrong D, Sepkowitz KA. Rotavirus outbreak on a pediatric oncology floor: possible association with toys. Am J Infect Control. Oct 2000;28(5):378-80. [Medline].

  46. Bass DM. Rotavirus and other agents of viral gastroenteritis. In: Behrman RE, Kliegman RM, Jenson HB, eds. Nelson Textbook of Pediatrics. 2004:1081-3.

  47. Bines JE. Rotavirus vaccines and intussusception risk. Curr Opin Gastroenterol. Jan 2005;21(1):20-5. [Medline].

  48. Hamilton JR. Viral enteritis. Pediatr Clin North Am. Feb 1988;35(1):89-101. [Medline].

  49. Kombo LA, Gerber MA, Pickering LK, Atreya CD, Breiman RF. Intussusception, infection, and immunization: summary of a workshop on rotavirus. Pediatrics. Aug 2001;108(2):E37. [Medline].

  50. Peter G, Myers MG. Intussusception, rotavirus, and oral vaccines: summary of a workshop. Pediatrics. Dec 2002;110(6):e67. [Medline].

  51. Pickering LK, Cleary TG. Infections of the gastrointestinal tract. In: Gershon AA, Hotez PF, Katz SL, eds. Krugman's Infectious Diseases of Children. 2004:220-1.

  52. Ramig RF. Pathogenesis of intestinal and systemic rotavirus infection. J Virol. Oct 2004;78(19):10213-20. [Medline].

  53. Santosham M, Foster S, Reid R, et al. Role of soy-based, lactose-free formula during treatment of acute diarrhea. Pediatrics. Aug 1985;76(2):292-8. [Medline].

  54. [Best Evidence] Vesikari T, Matson DO, Dennehy P, et al. Safety and efficacy of a pentavalent human-bovine (WC3) reassortant rotavirus vaccine. N Engl J Med. Jan 5 2006;354(1):23-33. [Medline].

  55. Widdowson MA, Bresee JS, Gentsch JR, Glass RI. Rotavirus disease and its prevention. Curr Opin Gastroenterol. Jan 2005;21(1):26-31. [Medline].

Previous
Next
 
Transmission electron micrograph of rotavirus. Image courtesy of Centers for Disease Control and Prevention.
Transmission electron micrograph of intact rotavirus particles, double-shelled. Image courtesy of Centers for Disease Control and Prevention and Dr. Erskine Palmer.
This electron micrograph reveals a number of RNA rotavirus virions, and a number of unknown, 29nm virion particles. Image courtesy of Centers for Disease Control and Prevention.
 
 
 
All material on this website is protected by copyright, Copyright © 1994-2012 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

DISCLAIMER: The content of this Website is not influenced by sponsors. The site is designed primarily for use by qualified physicians and other medical professionals. The information contained herein should NOT be used as a substitute for the advice of an appropriately qualified and licensed physician or other health care provider. The information provided here is for educational and informational purposes only. In no way should it be considered as offering medical advice. Please check with a physician if you suspect you are ill.