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CBRNE - Staphylococcal Enterotoxin B

  • Author: Bruce A Gleason, MD; Chief Editor: Duane C Caneva, MD, MSc  more...
Updated: Dec 31, 2015


Toxins are poisons produced by living organisms. Staphylococcal enterotoxin B (SEB) is an exotoxin excreted by the Staphylococcus aureus bacterium. Staphylococcus species thrive and produce toxins in unrefrigerated meats, dairy, and bakery products. SEB normally exerts its effect on the intestines and, therefore, is termed an enterotoxin. Not all toxins result in a lethal outcome, but they may result in significant morbidity.[1]

Staphylococcal enterotoxin B (SEB) is the toxin most commonly associated with classic food poisoning. It has also been demonstrated to cause a nonmenstrual toxic shock syndrome (TSS).[2] SEB has been studied as a potential biological warfare agent because it can easily be aerosolized; it is very stable; and it can cause widespread systemic damage, multiorgan system failure, and even shock and death when inhaled at very high dosages. However, SEB is classified as an incapacitating agent because in most cases aerosol exposure does not result in death but in a temporary, though profoundly incapacitating, illness lasting as long as 2 weeks.[3]


Staphylococcal enterotoxin B (SEB) is 1 of 7 originally identified enterotoxins produced by certain strains of the coagulase-positive S aureus bacteria, a gram-positive cocci that form clumps. Research has elucidated the structures of numerous enterotoxin-like superantigens, with two new enterotoxins, now known as SES and SET, just discovered in 2008.[4, 5, 6] S aureus is known to colonize the nasal passages and axillae in humans.


Staphylococcal enterotoxin B (SEB) consists of 239 amino acid residues and has a molecular weight of 28 kd. It is 1 of the 6 least antigenically distinct enterotoxin proteins that have been identified (A, B, C, D, E, G). SEB has 2 distinct tightly "packed" domains that have a very complex tertiary structure. It is this compact structure that enables SEB to be highly resistant to proteases, including trypsin, chymotrypsin, and papain, which are all found in the intestinal lumen.


Staphylococcal enterotoxin B (SEB) is a relatively stable compound that is easily soluble in water. It is moderately resistant to temperature fluctuations and can withstand boiling at 100 º C for several minutes. In the freeze-dried state, SEB can be stored for more than a year. For aerosol exposures, the effective dose, or ED50 (dose capable of incapacitating 50% of the exposed human population), is 0.0004 mcg/kg, and the lethal dose, or LD50, is 0.02 mcg/kg.[7]



Mechanism of toxicity

Many of the effects of staphylococcal enterotoxin B (SEB) are mediated stimulation of T lymphocytes by the host's immune system. The toxin binds directly to the major histocompatability complex (MHC) class II proteins on target cells, subsequently stimulating the proliferation of large numbers of T lymphocytes. SEB is a "bacterial superantigen" because it can form a "bridge" between the MHC II on the antigen presenting cells and the T-cell receptors on both CD4 and CD8 T cells, thereby bypassing the normal antigen processing and presenting mechanism. This bridging effect causes the release of massive amounts of cytokines, specifically interleukin 2 (IL-2), tumor necrosis factor β (TNF-β), and interferons.

The cytokines not only cause a recruitment of additional inflammatory cells but there is a relative deficient activation of negative counter-regulatory feedback loops. Taken together, the body's own inflammatory response most likely mediates many of the toxic effects of SEB. Ingestion of SEB produces profound gastrointestinal (GI) symptoms, including anorexia, nausea, vomiting, and diarrhea, which are believed to be mediated through the release of cytokines from T cells in the lamina propria of the intestines. Animal studies have shown that the severe pulmonary edema associated with aerosol exposure is likely secondary to T-cell proliferation within the respiratory mucosa and not the toxin itself.[1, 3, 8]




United States

The actual incidence of SEB-related food poisoning is unknown; many cases are so mild that patients do not seek treatment. Additionally, diagnoses in the emergency department are usually presumptive, and a number of other diseases may mimic SEB-induced gastroenteritis.


The gastrointestinal form of staphylococcal enterotoxin B (SEB) toxicity, while potentially debilitating for short durations, is rarely fatal with adequate hydration.

No data are available regarding the mortality and morbidity of inhalational SEB exposure.


Very young and elderly persons are likely the most susceptible to a complicated course.

Contributor Information and Disclosures

Bruce A Gleason, MD Chief, Department of Emergency Medicine, William Beaumont Army Medical Center, Ft Bliss, Texas

Bruce A Gleason, MD is a member of the following medical societies: American College of Emergency Physicians, Emergency Medicine Residents' Association

Disclosure: Nothing to disclose.


Kermit D Huebner, MD, FACEP Research Director, Carl R Darnall Army Medical Center

Kermit D Huebner, MD, FACEP is a member of the following medical societies: Alpha Omega Alpha, American Academy of Emergency Medicine, Association of Military Surgeons of the US, Society for Academic Emergency Medicine, Society of United States Air Force Flight Surgeons

Disclosure: Nothing to disclose.

Specialty Editor Board

Francisco Talavera, PharmD, PhD Adjunct Assistant Professor, University of Nebraska Medical Center College of Pharmacy; Editor-in-Chief, Medscape Drug Reference

Disclosure: Received salary from Medscape for employment. for: Medscape.

Chief Editor

Duane C Caneva, MD, MSc Senior Medical Advisor to Customs and Border Protection, Department of Homeland Security (DHS) Office of Health Affairs; Federal Co-Chair, Health, Medical, Responder Safety Subgroup, Interagency Board (IAB)

Disclosure: Nothing to disclose.

Additional Contributors

Jerry L Mothershead, MD Medical Readiness Consultant, Medical Readiness and Response Group, Battelle Memorial Institute; Advisor, Technical Advisory Committee, Emergency Management Strategic Healthcare Group, Veteran's Health Administration; Adjunct Associate Professor, Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences

Jerry L Mothershead, MD is a member of the following medical societies: American College of Emergency Physicians, National Association of EMS Physicians

Disclosure: Nothing to disclose.


The authors and editors of Medscape Reference gratefully acknowledge the contributions of previous author, Danielle M Pesce, DO, to the development and writing of this article.

  1. Woods JB, Darling RG, Dembek Z, et al. USAMRIID Medical Management of Biological Casualties Handbook. 6th ed. April 2005.

  2. CDC. Toxic-shock syndrome, United States, 1970-1982. MMWR Morb Mortal Wkly Rep. 1982 Apr 30. 31(16):201-4. [Medline].

  3. Ulrich RG, Sidell S, Taylor TJ. Staphylococcal enterotoxin B and related pyogenic toxins. Textbook of Military Medicine. Part I. Warfare, Weaponry and the Casualty. 1997. Vol 3: 621-631.

  4. Ono HK, Omoe K, Imanishi K, Iwakabe Y, Hu DL, Kato H, et al. Identification and characterization of two novel staphylococcal enterotoxins, types S and T. Infect Immun. 2008 Nov. 76(11):4999-5005. [Medline]. [Full Text].

  5. Kashiwada T, Kikuchi K, Abe S, Kato H, Hayashi H, Morimoto T, et al. Staphylococcal enterotoxin B toxic shock syndrome induced by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). Intern Med. 2012. 51(21):3085-8. [Medline].

  6. Yu J, Oh MH, Park JU, Myers AC, Dong C, Zhu Z, et al. Epicutaneous exposure to staphylococcal superantigen enterotoxin B enhances allergic lung inflammation via an IL-17A dependent mechanism. PLoS One. 2012. 7(7):e39032. [Medline]. [Full Text].

  7. Papageorgiou AC, Tranter HS, Acharya KR. Crystal structure of microbial superantigen staphylococcal enterotoxin B at 1.5 A resolution: implications for superantigen recognition by MHC class II molecules and T-cell receptors. J Mol Biol. 1998 Mar 20. 277(1):61-79. [Medline].

  8. Mattix ME, Hunt RE, Wilhelmsen CL, Johnson AJ, Baze WB. Aerosolized staphylococcal enterotoxin B-induced pulmonary lesions in rhesus monkeys (Macaca mulatta). Toxicol Pathol. 1995 May-Jun. 23(3):262-8. [Medline].

  9. Tierney LK, McPhee SJ, Papadakis MA. Current Medical Diagnosis and Treatment. 1998.

  10. Rajagopalan G, Sen MM, Singh M, Murali NS, Nath KA, Iijima K, et al. Intranasal exposure to staphylococcal enterotoxin B elicits an acute systemic inflammatory response. Shock. 2006 Jun. 25(6):647-56. [Medline].

  11. Rajagopalan G, Smart MK, Patel R, David CS. Acute systemic immune activation following conjunctival exposure to staphylococcal enterotoxin B. Infect Immun. 2006 Oct. 74(10):6016-9. [Medline]. [Full Text].

  12. Rao R, Nagarkatti P, Nagarkatti M. Role of miRNA in the regulation of inflammatory genes in staphylococcal enterotoxin B-induced acute inflammatory lung injury and mortality. Toxicol Sci. 2015 Apr. 144 (2):284-97. [Medline].

  13. Gholamzad M, Khatami MR, Ghassemi S, Vaise Malekshahi Z, Shooshtari MB. Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip. Jundishapur J Microbiol. 2015 Sep. 8 (9):e26793. [Medline].

  14. Khan AS, Cao CJ, Thompson RG, Valdes JJ. A simple and rapid fluorescence-based immunoassay for the detection of staphylococcal enterotoxin B. Mol Cell Probes. 2003 Apr-Jun. 17(2-3):125-6. [Medline].

  15. Virtual Naval Hospital, US Army Medical Research Institute of Infectious Diseases. Medical Management of Biological Casualties Handbook. 1998.

  16. Mantis NJ. Vaccines against the category B toxins: Staphylococcal enterotoxin B, epsilon toxin and ricin. Adv Drug Deliv Rev. 2005 Jun 17. 57(9):1424-39. [Medline].

  17. Yang M, Kostov Y, Bruck HA, Rasooly A. Carbon nanotubes with enhanced chemiluminescence immunoassay for CCD-based detection of Staphylococcal enterotoxin B in food. Anal Chem. 2008 Nov 15. 80(22):8532-7. [Medline]. [Full Text].

  18. Soto CM, Martin BD, Sapsford KE, Blum AS, Ratna BR. Toward single molecule detection of staphylococcal enterotoxin B: mobile sandwich immunoassay on gliding microtubules. Anal Chem. 2008 Jul 15. 80(14):5433-40. [Medline].

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