Donath-Landsteiner Hemolytic Anemia Workup
- Author: Trisha Simone Tavares, MD, FAAP; Chief Editor: Max J Coppes, MD, PhD, MBA more...
The diagnosis of Donath-Landsteiner hemolytic anemia (DLHA) can be elusive. The clinician must have a high index of suspicion.
Laboratory testing is crucial to making the diagnosis, and the medical team should be familiar with the assays available in the treating facility's laboratory.
Complete Blood Cell Count and Peripheral Smear
Anemia may be mild, moderate, or severe. Anemia is usually normocytic and normochromic. However, the mean corpuscular volume (MCV) may be elevated when reticulocytosis is present. Reticulocytosis does not develop until later in the disease; in the early stages, reticulocytopenia is often observed.
Leukopenia with or without neutropenia may be present early, but the leukocyte count gradually improves to within the reference range or higher. Leukocytosis may be present during an acute hemolytic episode.
The peripheral blood smear exhibits spherocytosis, polychromasia, nucleated RBCs, anisocytosis, poikilocytosis, and sometimes erythrophagocytosis by neutrophils (see the images below).[17, 19]
Blood typing should be performed on all patients even if anemia is mild. This is important because the hemoglobin may fall acutely and transfusion may be needed. Often, the presence of autoantibodies can interfere with blood typing. The D-L autoantibody may react with the RBCs of potential donors, making detection of alloantibodies difficult. Compatibility testing can be improved by performing typing at 37°C.
Direct Antiglobulin Test
The direct antiglobulin test (DAT) with anti-IgG usually produces negative results because of dissociation of IgG from the RBC surface at warm temperatures. The DAT may produce weakly positive results if run at a cold temperature. A positive DAT result is due to C3.
Indirect Antiglobulin Test
The indirect antiglobulin test, if performed, must be carried out at a cold temperature.
Assays using labeled monoclonal anti-immunoglobulin (Ig) G are preferable. The autoantibody is IgG with anti-P specificity, although the specificity may be for other antigens. The titer is usually less than 1:100.
Donath-Landsteiner Bithermic Hemolytic Test
This is a hemolytic assay in which the patient's serum is incubated with normal RBCs and complement at 0-4°C to allow the early components of complement to be fixed. Subsequently, the specimen is incubated at 37°C in order to allow the later components of complement to be activated. The membrane attack complex lyses the RBCs.
The procedure for this test is as follows:
Incubate normal papainized group O RBCs (papainization exposes RBC membrane P -antigen sites) and normal serum (as complement source) with the patient's serum (containing D-L antibody) at 0-4°C; the use of papainized pooled O-cells results in exposure of more antigen sites on the cell membrane to the antibody.
Incubate a second group of samples, as above, at 37°C
Incubate a third group first at 0-4°C for 30 minutes, then at 37°C for 60 minutes
The presence of hemolysis in the third group without hemolysis in either the first or second group of samples constitutes a positive test result and a diagnosis of Donath-Landsteiner hemolytic anemia.
The Donath-Landsteiner test has a low sensitivity, and results are positive only when the serum antibody titer is high and the test is performed precisely.
A false negative test may be seen if the following is present:
Low level of antibody due to consumption during hemolysis
Low complement levels from consumption during hemolysis (this can be prevented by mixing the patient sample with fresh normal donor serum as a source of complement)
If donor serum as a source of complement is added, presence of globoside in the serum may neutralize the Donath-Landsteiner (D-L) antibody 
Autoantibody specificity may be indicated if D-L test result is positive. Most cases are associated with anti-P specificity, but other specificities have been reported.
Testing should also be performed to determine whether the D-L antibody is IgG or IgM class.
If the D-L test is negative and there is a high index of suspicion, the negative result may be false. More sensitive tests exist. Discuss what other assays may be available in the local laboratories.
Blood Chemistry and Serology
Anemia may be mild, moderate, or severe.
Complement levels are typically decreased.
On serologic testing, results for syphilis, mycoplasmal infection, or viruses (eg, influenza A, measles, mumps, adenovirus, cytomegalovirus, varicella, Epstein-Barr virus [EBV]) may be positive, depending on the underlying cause.
Peripheral Blood Smear
Microscopic examination of the peripheral blood may show spherocytosis, RBC agglutination and anisopoikilocytosis. Erythrophagocytosis by neutrophils may also be seen.
Reticulocytosis is common. If the etiology of the condition is parvovirus B-19 infection, reticulocytopenia may occur.
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