Ophthalmologic Manifestations of Escherichia Coli Workup

Updated: Feb 05, 2016
  • Author: Donny W Suh, MD, FAAP; Chief Editor: Hampton Roy, Sr, MD  more...
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Workup

Laboratory Studies

The following studies are indicated in patients with suspected E coli infection:

  • CBC count with cell differentials

  • Blood agar

  • Sabouraud dextrose agar without cycloheximide

  • Thioglycolate broth

  • Chocolate agar

  • Löwenstein-Jensen medium

  • Nonnutrient agar with E coli overlay

  • Slides - Gram stain, Giemsa stain

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Imaging Studies

CT scanning of the orbit and head may be helpful in case of trauma to look for a foreign body.

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Procedures

With a corneal ulcer, anesthetize the cornea with topical drops, and, at the slit lamp, scrape the base and the leading edge of the infiltrate with the Kimura spatula, and place on the culture medium or slide.

With endophthalmitis, diagnostic and therapeutic vitrectomy is used to confirm the diagnosis and to evaluate the organisms' sensitivity to antibacterial agents.

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Histologic Findings

E coli is a gram-negative rod. E coli has a fermentative and respiratory type of metabolism.

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