17-Hydroxyprogesterone, Urine

Updated: Jun 23, 2022
  • Author: Muhammad Bader Hammami, MD; Chief Editor: Daniela Hermelin, MD  more...
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Reference Range

The reference range of urinary 17-hydroxyprogesterone (17-OHPG) in children is as follows: [1, 2, 3]

  • Age 3 days to 1 year: Up to 50 ng/24 hours

  • Age 1-8 years: Up to 300 ng/24 hours

The following is the reference of urinary 17-OHPG in adults:

  • Males: Up to 2 µg/24 hours

  • Females: Up to 4.5 µg/24 hours



Elevated urinary 17-hydroxyprogesterone (17-OHPG) levels are associated with the following:


Collection and Panels

Specifics for collection and panels are as follows:

  • Specimen type: Urine

  • Collection method: 24-hour urine collection (see image below)

    Urine sample. Urine sample.
  • Specimen volume: 10 mL

  • Other instructions: Record duration and total volume




An elevated level of 17-OHPG, an intermediate in the synthesis of adrenocortical hormones, is the distinguishing characteristic of 21-hydroxylase (CYP21) deficiency, the most common form of CAH.

Urine 17-OHPG is also frequently elevated in patients with PCOS or idiopathic hirsutism.


CAH is a general term for a group of inherited disorders in which a defect in cortisol biosynthesis results in overproduction of adrenocorticotropin hormone (ACTH), with secondary adrenal hyperplasia as a consequence. Classic CAH is generally recognized at birth or in early childhood because of ambiguous genitalia, salt wasting, or early virilization. Nonclassic adrenal hyperplasia is generally recognized at or after puberty because of oligomenorrhea or virilizing signs in females.

Indications for testing of urinary 17-OHPG include the following:

  • In a male child with premature sexual development

  • In females with hirsutism, other signs of virilization, oligomenorrhea, or infertility

A study by Itonaga et al indicated that the first morning value of pregnanetriol, a urinary metabolite of 17-hydroxyprogesterone, can be used in place of serum/blood 17-hydroxyprogesterone to monitor 21-hydroxylase deficiency, the most common type of CAH. The investigators found that, prior to glucocorticoid administration, a significant positive correlation exists between the first urinary pregnanetriol measurement and that of blood 17-hydroxyprogesterone. [4]


Patients should avoid use of corticosteroids, ACTH, estrogen, or gonadotropin medication for at least 48 hours before the start of urine collection.