Hypersensitivity Nephropathy Workup

Updated: Jan 19, 2022
  • Author: Micah L Thorp, DO, MPH; Chief Editor: Vecihi Batuman, MD, FASN  more...
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Laboratory Studies

Urine is often examined for eosinophils, but this method lacks adequate sensitivity and specificity. One study found a positive predictive value of 38%. [19] Regular urine microscopy must be supplemented with specific stains for eosinophils (Hansel stain). A proportion of patients develop peripheral blood eosinophilia, but this is an inconsistent feature. [11]

Urinalysis often reveals sterile pyuria with microscopic hematuria. Proteinuria is often present, but the quantity varies greatly, ranging from nephrotic levels in acute interstitial nephritis from nonsteroidal anti-inflammatory drugs to less than 1 g/d in others.


Imaging Studies

Renal ultrasound is of minimal value. Normal-sized kidneys with a slight increase in echogenicity are typically noted.

Gallium scans often show diffuse bilateral uptake. This can be helpful in differentiating acute interstitial nephritis and acute tubular necrosis, which has a uniformly negative scan result.



The criterion standard diagnostic test for acute interstitial nephritis is kidney biopsy. Unfortunately, little else is available to help make a definitive diagnosis. Biopsy frequently reveals either a diffuse or segmental mixed infiltrate (see the images below). 

Acute interstitial nephritis with mononuclear cell Acute interstitial nephritis with mononuclear cell infiltrate.
Mononuclear cell infiltrate between tubules. Mononuclear cell infiltrate between tubules.