Approach Considerations
No laboratory studies are specific for multicentric reticulohistiocytosis (MRH). Findings, however, are as follows:
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Elevated erythrocyte sedimentation rate (ESR) or anemia: In about 50% of patients
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Thyroid function abnormalities: In some, but not all, patients
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Diabetes: In about 6% of patients
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Hypercholesterolemia: Found in one third of patients; often associated with xanthelasma, but this seems to be unrelated to MRH
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Rheumatoid factor: Characteristically negative, but it has been reported to be positive in at least 4 cases
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Paraproteinemia, cold agglutinins, cryoglobulinemia, antinuclear antibody, or hypergammaglobulinemia: Occasionally found
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Synovial fluid abnormalities: Variable; reports exist of elevated counts of neutrophils or mononuclear cells.
Imaging Studies
Radiography
Routine radiographs of joints may be helpful. Changes, which may develop rapidly, are most commonly seen in the proximal or distal interphalangeal joints. Findings can include the following:
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Destruction of bone in affected joints is disproportionate to cartilage loss
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Lesions may resemble gouty erosions
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Mild osteopenia is noted
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Marked resorption of subchondral bone is common
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Cutaneous nodules may be seen as soft tissue swellings
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Findings on joint fluid analysis vary; effusions may demonstrate polymorphonuclear leukocytes or mononuclear cells
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Bilaterally symmetrical, sharply circumscribed erosions spread from the margins to the joint surfaces
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Separation of the bone ends often occurs, but no subchondral sclerosis and little or no periosteal reaction are seen
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Osteoporosis is mild or absent
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Erosions in osteoarthritis begin centrally rather than at the joint margins
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In psoriatic arthritis and reactive arthritis, erosions are asymmetrical and have poorly defined margins; the erosions are also associated with periosteal new bone formation
Other modalities
Magnetic resonance imaging (MRI) [53] and computed tomography (CT) scanning [54] have been reported to be helpful, but they are not needed in most cases.
Gallium scans and bone scans have been used in the past; however, recommending them routinely is difficult because of their nonspecificity. [55]
Histologic Findings
When the skin lesions are present, skin is the easiest site from which to obtain a biopsy specimen. Histiologic studies in MRH reveal the following (see the images below):
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Early skin lesions demonstrate a dermal lymphohistiocytic infiltrate
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Histiocytes may be oncocytic, small, or multinucleated
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Histiocytes may occasionally be lipid-laden, but are not usually foamy to the degree found in other histiocytic disorders
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Mature lesions demonstrate multinucleated giant cells, with a pale, fine, granular (ie, ground-glass) eosinophilic cytoplasm filling the dermis
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Periodic acid-Schiff stain results are positive and are diastase resistant
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Lipid stain results may be positive
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Results of acid phosphatase, nonspecific esterase, and lysozyme stains are usually positive
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S100 protein, CD34, factor XIIIa, and alpha-1-antitrypsin results are typically negative
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Histiocytes stain positive for vimentin, CD68, and CD45, and small activated histiocytes stain positive for MAC387 [2]
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One study demonstrated aberrant expression of CD10 (a 90- to 110-kd cell surface zinc-dependent metalloprotease) in skin and synovial multinucleated giant cells in patients with MRH, whereas expression of CD10 was not detected in other histiocytic lesions, including skin samples of granuloma annulare, giant-cell tendon sheath tumor, ruptured epidermoid cyst, sarcoidosis, xanthogranuloma, and synovial samples of rheumatoid arthritis and pigmented villonodular synovitis [3]
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Langerhans granules are absent on electron microscopy
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In a synovial biopsy, lipid-laden giant cells and histiocytes are similar to those seen on skin biopsy; histiocytes are sometimes found after blind synovial biopsies in patients who have unclassified arthritis but no skin lesions [4]
Histopathology of multicentric reticulohistiocytosis (MRH) skin lesions. Higher power demonstrating multinucleated giant cells with eosinophilic ground-glass cytoplasm.
Low-power view of a biopsy sample of large histiocytes and multinucleated giant cells in the dermis.
In addition to macrophage properties, the histiocytes may have osteoclastic properties. Those of the osteoclast phenotype express osteoclast selective markers, tartrate-resistant acid phosphatase, and cathepsin K, and they respond to treatment with bisphosphonates. [3]
Stains
The cytoplasm of the histiocytes stains with the periodic acid-Schiff (PAS) stain. Although positive staining with Sudan black B and scarlet red indicates the presence of lipid within these cells, they are not usually foamy to the degree found in many other histiocytic disorders.
The cells stain with the usual macrophage markers, such as lysozyme (see the image below), CD68, MAC387, and human alveolar macrophage-56 (HAM-56), as well as CD10 (multinucleated giant cells only). [3] Staining results for S-100, CD1a, CD34, factor XIIIa, or alpha-1-antitrypsin are typically negative.
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Multiple erythematous nodules are present on the dorsal hands of this adolescent with an inflammatory arthropathy.
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Multiple erythematous to brown nodules on the fingers.
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Erythematous, poikilodermatous, mamillated plaque on the anterior chest.
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Histopathology of multicentric reticulohistiocytosis (MRH) skin lesions.
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Histopathology of multicentric reticulohistiocytosis (MRH) skin lesions. Higher power demonstrating multinucleated giant cells with eosinophilic ground-glass cytoplasm.
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Erythematous to brown papules overlying the right dorsal hand and wrist and erythematous to violaceous patches over the right dorsal hand and fingers. The cutaneous changes are in a photodistributed pattern and mimic the changes of dermatomyositis.
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Erythematous to brown papules and erythematous to violaceous patches overlying the right dorsal hand and fingers, with a crusted erosion overlying the fourth metacarpophalangeal joint. These cutaneous changes can be easily confused with dermatomyositis.
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Nodules on a hand with deformed joints from arthritis due to multicentric reticulohistiocytosis.
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Swollen elbow and nodules on the forearm.
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Cobblestone papules on the eyelid and papules on the forehead.
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Low-power view of a biopsy sample of large histiocytes and multinucleated giant cells in the dermis.
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High-power view of large histiocytes in the dermis.
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Brown immunoperoxidase staining for lysozyme in histiocytes.
