Thymic Epithelial Neoplasms Imaging

Thymic tumors occupy the anterior mediastinum, which is immediately posterior to the sternum and the anterior surface of the pericardium and great vessels. Thymic epithelial neoplasms or tumors, also known as thymoma and thymic carcinoma, are the most common primary neoplasms of the anterior mediastinum. The most widely used classification system is the World Health Organization (WHO) classification, based on the morphology of the epithelial cells and ratio of the lymphocytes to epithelial cells. The Masaoka-Koga classification describes the extent and spread of thymic epithelial malignancies. Tumors of thymic, lymphatic, or germ cell origin most commonly occur in this compartment, although aberrant parathyroid or thyroid tissue masses are sometimes found, along with vascular and mesenchymal tissue masses. Approximately two thirds of all mediastinal tumors and cysts are symptomatic in children, whereas only a third are symptomatic in adults. When all age groups are considered, nearly 55% of patients with benign mediastinal masses are asymptomatic at presentation, compared with only approximately 15% of those in whom masses are found to be malignant. ^{[1, 2, 3, 4, 5, 6]}

Preferred examination

Conventional radiology is the first imaging technique to perform when an anterior mediastinal mass is suspected. A large anterior mediastinal mass is readily identified by chest radiography, as it typically appears as an extra soft tissue mass or opacity. Although the density of a soft tissue mass is similar to anterior mediastinal structures, the silhouette sign, in which there is a loss of normal anatomical borders, helps make the diagnosis. Identifying a small mediastinal mass is more difficult. The presence of an anterior junction line, which represents the point of contact between the anterior lungs and their pleural surfaces anterior to the cardiovascular structures, can help exclude the presence of an anterior mediastinal mass; however, this line is seen in only 20% of normal chest radiographs.

Computed tomography (CT) scanning is most valuable in the diagnosis of thymic lesions and anterior mediastinal masses. Magnetic resonance imaging (MRI) may also be used. Routine chest CT and MRI can effectively identify thymoma, but neither is reliable to differentiate between thymic hyperplasia and normal thymus in patients with myasthenia gravis. Standard imaging for thymic tumors is IV contrast-enhanced CT of the thorax, which provides a complete exploration of the mediastinum and pleura from the apex to the costodiaphragmatic recesses. ^{[7, 8, 1, 2, 9, 10, 11, 12, 13, 14]}

The eighth edition of the TNM classification of malignant tumors includes an official staging system for thymic epithelial tumors recognized by the American Joint Committee on Cancer (AJCC) and the Union for International Cancer Control (UICC). Thymic epithelial tumors with clearly invasive or metastatic features seen on CT are associated with having higher AJCC/UICC pathologic TNM stage. However, features of lobulated contour and heterogeneous internal density are also associated with higher-stage disease. ^{[8, 10]}

(See the images below.)

About one half of all thymic tumors are malignant in individuals aged 20-40 years, and one third are malignant in persons younger than 20 years and those older than 40 years. (A malignant thymic tumor is seen in the image below.)

 

Many thymomas may be visualized using routine chest radiographs in posteroanterior (PA) and lateral views. A thymoma frequently appears as a smooth, lobulated mass in the superior aspect of the anterior mediastinum, often projecting into one of the hemothoraces. The mass may be calcified or cystic. In most cases, thymolipomas are detected incidentally on chest radiographs. They are often large, ranging up to 36 cm in diameter (mean, 18 cm) at the time of diagnosis. Thymolipomas may project into the hemithoraces; they may be seen draping over the heart and extending as far as the costophrenic angles. They can be easily mistaken for pleural or pericardial tumors or even pulmonary sequestration.

(See the images below.)

 

From birth to puberty, CT scan attenuation of the thymus is comparable to that of the chest wall musculature. The outer contours of the thymus may be convex laterally. The lobes, though separate, may have a triangular shape and may be slightly rotated to the left. The junction between the lobes is about 2 cm to the left of the midline.

From puberty to 30 years of age, the overall attenuation value diminishes secondary to fatty infiltration; during this period, attenuation is less than that of skeletal muscle. The thymus is often seen as a discrete triangular or bilobed structure, with the outer borders appearing straight or slightly concave laterally.

After the age of 30 years, remnants of thymic tissue appear as small islands of soft tissue attenuation; these islands appear as linear, oval, or small and round configurations on a background of more abundant fat.

In the older patient, only a thin fibrous skeleton of the thymus remains. At this point, the thymus is almost totally composed of fat.

The most reliable and meaningful measurements of the thymus are related to its thickness. Before the age of 20 years, 1.8 cm is the normal thickness; thereafter, the normal thymus does not exceed 1 cm in thickness.

(Changes in the thymus over time are demonstrated in the image below.)

 

Thymic hyperplasia

CT scanning may show symmetrical, diffuse enlargement of the thymus. When the enlargement is asymmetrical, it may mimic a thymoma; thymoma should be considered in the differential diagnosis. ^[15]

About two thirds of patients with myasthenia gravis have thymic hyperplasia; 25-50% of these patients are likely to have a normal thymus on CT scanning. Histologic analysis of the thymic medulla reveals numerous lymphoid follicles with active germinal centers.

Mature teratoma

On CT, teratomas are sharply defined; low-attenuation cystic components predominate. Fatty tissue is seen in about 50% of cases, and fat-fluid levels have been reported. Foci of calcification and ossification are commonly seen; in addition, soft tissue attenuation may be depicted. In rare cases, the tumor may erode and rupture into adjacent structures, such as the lung, tracheobronchial tree, and pleural space. (See the image below.)

Seminoma

On CT scans, seminomas are usually large and homogeneous, with soft tissue attenuation. Areas of low attenuation often are present secondary to necrosis and hemorrhage.

Nonseminomatous tumor

Nonseminomatous tumors include embryonal carcinoma, endodermal sinus tumor, choriocarcinoma, and mixed germ cell tumor. On CT scans, these lesions are often large and heterogeneous, with large (>50%) areas of low attenuation. They may contain areas of calcification.

Thymic lymphoma

On CT scans, the thymus is enlarged, symmetrically or asymmetrically; this enlargement often makes it difficult to differentiate the thymus from the enlarged surrounding lymph nodes. At times, differentiating the thymus from a thymoma is difficult, although the clinical picture and the presence of other sites of lymphadenopathy are often helpful in the diagnosis.

(See the image below.)

Thymic carcinoma

Thymic carcinoma belongs to a group of uncommon epithelial neoplasms. They are characterized by cytologic atypia and anaplasia. On CT scans, they often show central necrosis in a large tumoral mass, with invasion and infiltration of adjacent structures in the mediastinum. Because of their aggressive nature, they are likely to produce hematogenous and lymphatic spread, locally and distally.

Preoperative CT imaging features may help identify patients at risk for incomplete surgical resection of thymic carcinoma. A retrospecitive review of  preoperative CT findings of 41 patients with thymic carcinoma found that in addition to lesion size, the feature associated with incomplete surgical resection was the degree of tumor contact with adjacent mediastinal vessels on the preoperative computed tomography image (P=0.038). ^[16]

Thymolipoma

CT scanning reveals a fatty mass interspersed with varying amounts of thymic soft tissue. The whole mass often consists of adipose tissue except for a thin rim of thymic tissue; this finding is consistent with soft tissue attenuation and mimics a pure lipoma of the mediastinum.

(See the image below.)

Thymic cyst

On CT scans, a thymic cyst appears homogeneous with water attenuation. The attenuation may vary, depending on the contents of the cyst. High attenuation may be present if the cyst contains proteinaceous fluid or blood from hemorrhage. A neoplasm with cystic degeneration may closely mimic a thymic cyst; associated soft tissue attenuation may help in their differentiation.

Thymic rebound

After initiation of chemotherapy, CT scans may reveal a decrease in the size of the thymus. Rebound in the form of overgrowth occurs a few months after completion of chemotherapy. Criteria for rebound include an increase in size of greater than 50%, as compared with the baseline volume.

Degree of confidence

Chest CT scanning performed with and without contrast enhancement is clearly superior to routine radiography. A small thymic tumor can easily be missed on a chest radiograph, whereas CT scans distinctly delineate the tumor. In addition, enhancement often helps in clearly differentiating the mass from the surrounding vascular structures. This is helpful in planning surgery. ^{[8, 9, 11, 12, 13]}

On imaging studies, bilateral extension of the mass, absence of fat planes, and invasion of adjacent structures are indications of malignancy. MRI may show similar findings. The demonstration of encapsulation of the mass and the homogeneous enhancement of the capsule are indications of benign tumors; these structures are better imaged with CT scanning. MRI and CT scanning often complement each other and can facilitate preoperative diagnosis and staging of the thymus neoplasms.

In cases of lymphoid follicular hyperplasia, the thymus may not always appear enlarged; this may be overlooked on CT scans.

False positives/negatives

CT scanning is highly sensitive for thymomas. If the thymus appears grossly asymmetrical or if it has a lobular configuration, the diagnosis of a thymoma should be strongly considered. However, thymomas cannot be distinguished from other thymic masses on CT scans unless fat is visible. Thymomas are usually homogeneous and show mild enhancement with the use of contrast. They tend to grow to one side of the mediastinum or the other. (Images of thymomas appear below.) ^[17]

When cystic degeneration, necrosis, and old hemorrhage are present, the affected areas are of low attenuation. Normal thymus that has undergone fatty infiltration and a pulmonary mass adjacent to the anterior mediastinum sometimes mimic a thymoma. In cases of encapsulated thymoma, CT scanning often reveals a preserved fat plane completely surrounding the mass. Fibrous adhesions and inflammation may mimic invasion of the tumor.

On CT scans, invasive thymoma is often characterized by encasement of mediastinal structures and pericardial/pleural implants; in advanced cases, transdiaphragmatic spread is seen.

In some cases, MRI is superior to CT scanning for visualizing the thymus and for differentiating it from the surrounding soft tissue. In healthy children younger than 5 years, MRI shows the thymus to have a quadrilateral shape and biconvex lateral contours. ^[18] In older children and adolescents, the thymus is triangular with straight, lateral margins. On T1-weighted images, the thymus appears homogeneous with a signal intensity slightly greater than that of muscle; on T2-weighted images, the signal intensity is close to that of fat.

Mass lesions in the mediastinum have sufficiently different imaging characteristics to allow their distinction on MRI from normal structures and fat, and MRI produces excellent cross-sectional images in the mediastinum without contrast enhancement; with CT, contrast material is often needed to properly identify a mass and to avoid mistaking blood vessels for a mass lesion.

Encasement or invasion of the vasculature, esophagus, and trachea and involvement of the pericardium, myocardium, and pleura are accurately detected with MRI.

In all adults, the thymus is visible on MRI. Distinction between higher intensity mediastinal fat and the relatively hypointense thymus is optimal on T1-weighted images because of the long T1 of the thymus. The progressive decrease in T1 with advancing age is commensurate with the fatty infiltration associated with advancing age. However, the T2 relaxation times of the thymus do not change with aging. The thymus usually appears thicker on MRI than on CT in patients older than 20 years.

On T1-weighted images, thymomas have medium signal intensity that is higher than that of skeletal muscle but that is lower than that of fat. On T2-weighted images, the signal intensity approaches or exceeds that of fat. The mass often appears mostly homogeneous; areas of cystic degeneration appear as areas of variable signal intensity on T1-weighted images. This appearance is the result of variations in protein content or of hemorrhage; however, on T2-weighted images, thymomas appear bright. The hypointense fibrous septa often gives the mass a lobulated appearance.

Thymic hyperplasia

Thymic hyperplasia and normal thymus share the same characteristics on MRI. In the case of thymolipoma, T1-weighted MRIs reveal high signal intensity, which represents fat; strands of intermediate intensity represent thymic tissue.

Thymic cyst

T1-weighted MRIs of thymic cysts reveal low signal intensity; T2-weighted images show high signal intensity consistent with the fluid component of the lesion. T1-weighted images naturally show high signal intensity if the cyst contains blood from hemorrhage or if it is rich in proteinaceous fluid.

Qualitative evaluation of gross thymic morphology (ie, size, shape, margins, and signal intensity) is usually sufficient for distinguishing normal thymus from abnormal thymus. The abnormal thymus generally is enlarged, multilobular, or inhomogeneous because of the presence of cystic degeneration, hemorrhage, septations, fibrosis, or calcification, as seen on pathologic sections. In patients with lymphoma, associated lymphadenopathy is helpful in distinguishing normal thymus from abnormal thymus.

On T1-weighted spin-echo images, thymic lipomas have areas of high signal intensity, because of their fat content; the signal intensity is similar to that of subcutaneous fat, with areas of intermediate signal intensity reflecting the presence of soft tissue. Although thymic lipomas can attain a large size, they invariably do not invade surrounding structures. However, they can cause mass effect on the surrounding structures because of their size.

MRI findings in thymic carcinoid tumors are nonspecific and are identical to those of thymoma.

On T1-weighted images, thymic carcinoma has higher signal intensity than muscle; on T2-weighted images, there is an increase in signal. Heterogeneous signals often reflect the presence of necrosis, cystic degeneration, or hemorrhage.

Teratomas have various appearances on MRI, depending on the composition of the tumor. They commonly contain fat, which is of high signal intensity on T1-weighted images. Cystic changes may also be present; such changes have low signal intensity on T1-weighted images, but they have increased signal intensity on T2-weighting.

MRI often shows the inhomogeneous nature of seminomas.

Thymic carcinoid

Thymic carcinoids tend to be large masses with irregular contours, no capsule, heterogeneous intensity on T2-weighted images, and heterogeneous enhancement and local invasion on CT or MRI. A necrotic or cystic component is often seen in atypical carcinoids. These radiologic features mimic those of high-risk thymomas and/or thymic carcinomas. ^[19]

Lymphoma and residual tumor

The MRI signal characteristics of untreated lymphomas are different from those of treated lymphomas. Untreated lymphomatous tissue has high signal intensity, whereas a homogeneous, hypointense pattern is characteristic of inactive residual fibrotic masses in patients receiving successful therapy for lymphoma. A heterogeneous pattern with mixed hypointensity and hyperintensity is often seen in untreated nodular sclerosing Hodgkin disease. A heterogeneous pattern with mixed areas of low and high signal intensity on T1- and T2-weighted images is seen in lesions containing mixed fat (high signal intensity) and fibrous tissue (low signal intensity). This pattern is seen after treatment of patients with sterilized tumors. Positron emission tomography (PET) CT may play a role in assessing for residual lymphoma. ^{[20, 21, 22, 23, 24, 25]}

Degree of confidence

With MRI of the thorax, motion artifacts may occur. Breathing motion and pulsation of the heart and great vessels can markedly degrade image quality. Hence, in general, fast imaging sequences and artifact reduction techniques must be used for MRI of the mediastinum and chest.

MRI provides information similar to that provided by CT scanning in the evaluation of thymomas. MRI is particularly useful when an intravenous contrast agent cannot be administered for use with CT because the patient is allergic to the agent.

FDG-PET/CT has been found to provide useful information for differentiating epithelial tumors. ^{[15, 17, 20, 21, 23, 24, 25, 26, 27]} In a study by El-Bawab et al, FDG-PET/CT appeared to be more accurate than conventional imaging in distinguishing recurrent tumors in the mediastinum from fibrotic scar tissue in patients who underwent surgical excision of a thymoma, with all mediastinal recurrences (N=11) being correctly identified by FDG-PET/CT. ^[17]

Thallium-201 (²⁰¹Tl) single photon emission CT (SPECT) scanning is useful for the evaluation of thymic lesions associated with myasthenia gravis, including lymphoid follicular hyperplasia and thymoma. ^[28]

On early images, ²⁰¹Tl accumulation is more intense in thymomas than in the normal thymus and in lymphoid follicular hyperplasia. On delayed images, uptake is more intense in thymoma and in lymphoid follicular hyperplasia than in the normal thymus. Therefore, ²⁰¹Tl SPECT scanning can be used to differentiate normal thymus from lymphoid follicular hyperplasia and thymoma in patients with myasthenia gravis.

Thallium-201 uptake is considered to reflect various factors, including cellular metabolic activity, regional blood flow, and the number of viable cells in the lesion; hence,²⁰¹ Tl imaging is in some respects superior to CT scanning.