Pediatric Crohn Disease Workup

Updated: Oct 16, 2017
  • Author: Andrew B Grossman, MD; Chief Editor: Carmen Cuffari, MD  more...
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Laboratory Studies

Laboratory data for Crohn disease are nonspecific. The complete blood count (CBC) may reveal evidence of hypochromic microcytic anemia due to the iron deficiency anemia secondary to gastrointestinal (GI) blood loss, or it may reveal normocytic anemia due to the anemia of chronic disease.

levels of acute-phase reactants, the erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels are often elevated in patients with Crohn disease. However, a normal ESR or CRP level should not deter further evaluation in a suspicious case.

Hypoalbuminemia is a common laboratory finding in patients with Crohn disease. Additional common deficiencies include iron and micronutrients (eg, folic acid, vitamin B-12, serum iron, total iron binding capacity, calcium, and magnesium).

Stool studies should be obtained to rule out bacterial or parasitic infection.

Serologic testing for inflammatory bowel disease (IBD) is available. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies to anti– Saccharomyces cerevisiae (ASCA) have been associated with Crohn disease, whereas perinuclear antineutrophil cytoplasmic antibody (p-ANCA) has been associated with ulcerative colitis (UC).

Although these tests might assist in differentiating between Crohn disease and UC, they are not good screening tests. In a retrospective review, serologic screening that included ASCA, pANCA, and antibody to Escherichia coli outer membrane porin (anti-OmpC) demonstrated a sensitivity of 60%, a specificity of 91%, and a positive predictive value of 60%. [16]

Excretion of fecal calprotectin, a protein derived from neutrophils, is increased with colorectal inflammation. [1] Enzyme-linked immunosorbent assay (ELISA) for fecal calprotectin is available; the cutoff level is 50 µg/g feces.


Radiography, MRI, CT, US, and PET

A single-contrast upper GI radiologic series with small-bowel follow-through (SBFT) can be used to evaluate the small intestine, which cannot be reached during endoscopy (see the image below).

Image obtained during upper gastrointestinal serie Image obtained during upper gastrointestinal series with small-bowel follow-through shows narrowing and irregularity in distal ileum in 16-year-old male adolescent with Crohn disease.

Magnetic resonance enterography (MRE) [2] and computed tomography enterography (CTE) are increasingly being used for evaluation of the small bowel. Both modalities are as sensitive and specific as SBFT for detection of small bowel inflammation and may be more accurate for detection of extraenteric complications, including fistulae and abscesses. [3] MRE is a particularly attractive option because of the lack of radiation exposure.

Proximal small bowel Crohn disease is present in up to 35% of children with this chronic inflammatory bowel disorder. It is also regarded as a more severe clinical phenotype that is associated with significant morbidity, including poor growth. MRE has now become the radiological procedure of choice in identifying proximal small bowel disease in children with Crohn disease. Moreover, the increased sensitivity of MRE to detect early-onset disease may allow physicians to tailor therapy more effectively. [17]

MRI is especially useful in the evaluation of pelvic and perianal disease (see the images below). Abdominal ultrasonography (US) can be used to investigate intestinal disease and to rule out gallbladder and kidney stones. Positron emission tomography (PET) is an experimental diagnostic tool.

Inflamed terminal ileum in 10-year-old girl with C Inflamed terminal ileum in 10-year-old girl with Crohn disease.
Small abscess on right side of anal sphincter in 9 Small abscess on right side of anal sphincter in 9-year-old boy with Crohn disease.


The development of flexible, small-caliber endoscopes has allowed colonoscopic evaluation of pediatric patients of all ages, including infants. Colonoscopy with several colonic and terminal ileal biopsies is invaluable and considered a standard in the diagnosis of Crohn disease (see the image below).

Colonoscopic image of large ulcer and inflammation Colonoscopic image of large ulcer and inflammation of descending colon in 12-year-old boy with Crohn disease.

Upper endoscopy, or esophagogastroduodenoscopy (EGD), should be part of the first-line investigation in all new cases of suspected Crohn disease. It is useful in planning therapy and in differentiating between Crohn disease and UC, especially if granulomas are present. Clinically significant upper GI inflammation can be present in the absence of upper GI symptoms.

Video capsule endoscopy is increasingly being used to evaluate for small-bowel Crohn disease in children. [4] Before the procedure is initiated, a dissolvable patency capsule should be placed or small bowel imaging performed to ensure that there are no areas of narrowing or stricture where the video capsule might create an obstruction.


Histologic Findings

The microscopic findings in intestinal biopsy samples from pediatric patients with Crohn disease consist of edema, inflammation (mononuclear and polymorphonuclear), cryptitis and crypt abscesses, architectural crypt changes, and transmural extension of the inflammation (see the image below).

Histologic features of chronic colitis with crypt Histologic features of chronic colitis with crypt atrophy and branching and lymphocytic infiltrate. Hematoxylin-eosin staining. Image courtesy of Dr E Ruchelli.

The presence of granulomas may be helpful in differentiating between UC and Crohn disease (see the image below), but granulomas are present in only about 30% of biopsy specimens obtained from patients with Crohn disease.

Colonic granuloma in patient with Crohn disease. H Colonic granuloma in patient with Crohn disease. Hematoxylin-eosin staining. Image courtesy of Dr E Ruchelli.


Multiple scoring systems incorporating the patient’s history, physical findings, and laboratory data have been developed to assess disease activity in adults with Crohn disease.

The Pediatric Crohn Disease Activity Index (PCDAI) was developed and validated in 1990. Its results are correlated with the physician’s global assessment and with the modified Harvey-Bradshaw index, and it has significant interobserver reliability. The important difference between this index and the Crohn Disease Activity Index (CDAI), which was developed for use in adults with Crohn disease, is the inclusion of growth parameters in the score.